Lipase acrylic resin

Manufactured by Merck Group

Lipase acrylic resin is a laboratory equipment product designed for the purification and isolation of lipase enzymes. It is a solid-phase affinity chromatography medium that specifically binds to lipase proteins, allowing for their effective separation and concentration from complex biological samples.

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Lab products found in correlation

Glycerol, by Merck Group (1 mentions) Molecular sieves, by Merck Group (1 mentions) Tert butanol, by Thermo Fisher Scientific (1 mentions) Sebacic acid, by Merck Group (1 mentions) Deuterated acetone, by Merck Group (1 mentions) Acetone, by Labsynth (1 mentions)

Spelling variants of this product

Lipase (128 citations) Acrylic resin (2 citations) Lipase B acrylic resin (2 citations)

2 protocols using lipase acrylic resin

Lipase-catalyzed Synthesis of Chiral Acetyl Substrates

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Example 11

[Figure (not displayed)]

A solution of acetyl substrate was dissolved in ethyl ether (Avantor Performance Materials, Inc.; Lot No.: 0000128533) and 0.1 M pH 7.0 potassium phosphate buffer at room temperature. Lipase acrylic resin (Sigma-Aldrich, Inc.; Lot No. SLBW1544) was added into the solution. The reaction mixture was put at room temperature for 16 hours. The reaction mixture was filtered and concentrated by the rotatory evaporator to get crude. The residue was purified by column chromatography through a pad of silica gel (FUJI Silysia Chemical Ltd.; Cat No. HT80186). Eluent system with the column volume (EtOAc/Hexane=1/4 to 3/1). The collected fractions were concentrated by the rotatory evaporator and high-vacuum to afford the product. Table 14 indicated the production yield by using lipase reagent of acetyl substrates.

TABLE 14

Production yield by using lipase reagent of acetyl substrates

Reagent

Lipase from CALB

Substrate(Yield, ee %)

[Figure (not displayed)]
77%, 99% ee, R form

OBI-3424-6-OAc56%, 99% ee, R form

(R form major)

US11773118B2. Methods of making high enantioselective secondary alcohols (2023-10-03). OBI PHARMA, INC. [TW]. Inventors: Cheng-Der Tony Yu [TW], Yih-Huang Hsieh [TW], Shu-Yi Lin [TW], Chin-Sheng Chao [TW], Yin-Cheng Hsieh [TW], Ming-Tain Lai [TW].

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Enzymatic Esterification Optimization

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Sebacic acid (99.0%, Sigma-Aldrich),
lipase acrylic resin—CALB (lipase B from C.
antarctica, ≥5000 U g^–1,
Sigma-Aldrich), and deuterated acetone (99.9% atom D, Sigma-Aldrich)
were used without any treatment. Molecular sieves, 5 Å (spheres,
4–8 mesh, Sigma-Aldrich), were exhaustively washed with distilled
water and dried in an oven at 400 °C for 4 h. Glycerol (≥99.5%,
Sigma-Aldrich), tert-butanol (99.5%, Acros Organics),
acetone (≥99.5%, LabSynth), and acetonitrile (≥99.5%,
LabSynth) were kept under Molecular sieves. Tetrahydrofuran (≥99.0%,
Vetec) was dried using metallic sodium and benzophenone under reflux
and argon flow; this was followed by distillation and storage in a
closed bottle with Molecular sieves.

Perin G.B, & Felisberti M.I. (2020). Enzymatic Synthesis of Poly(glycerol sebacate): Kinetics, Chain Growth, and Branching Behavior. Macromolecules, 53(18), 7925-7935.

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