The primary antibodies of TEAD1 (sc-376113), TEAD4 (sc-134071), and CTGF (L-20) (sc-14939) were from Santa Cruz (Dallas, TX, USA). YAP1 (ab52771) antibody and HA tag (ab18181) were achieved from Abcam (Cambridge, MA, USA). AMOTL1 (HPA001196) and Flag-tag (F3165) antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other primary antibodies were from Cell Signaling (Danvers, MA, USA), including p21 (#2946), p27 (#2552), pRb (Ser807/811) (#9308), Erk (#9102), pErk (#9101), Myc (#2278), cyclin D1 (#2978), c-Myc (#9402), and GAPDH (#2118). Anti-Mouse IgG-HRP (Dako, Glostrup, Denmark, 00049039, 1:30,000) and anti-Rabbit IgG-HRP (Dako, 00028856, 1:10,000) were used for secondary antibodies. The related protocol was suggested before [13 (link)].
Yap1 ab52771 antibody
Manufactured by Abcam
Sourced in United States
YAP1 (ab52771) antibody is an antibody that targets the Yes-associated protein 1 (YAP1), a key regulator of the Hippo signaling pathway. The antibody can be used to detect and quantify the expression of YAP1 in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin d1, by Cell Signaling Technology (2 mentions)
Anti mouse igg hrp, by Agilent Technologies (2 mentions)
Anti rabbit igg hrp, by Agilent Technologies (2 mentions)
C myc, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
P rb ser807 811, by Cell Signaling Technology (2 mentions)
Amotl1 hpa001196, by Merck Group (1 mentions)
Flag tag f3165, by Merck Group (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Cyclin d3, by Cell Signaling Technology (1 mentions)
Cleaved parp asp214, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions)
Cleaved caspase 7 asp198, by Cell Signaling Technology (1 mentions)
2 protocols using yap1 ab52771 antibody
1
Antibody Sources for Cell Signaling
2
Western Blot Analysis Specifications
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Western blot analysis was performed in our previous study.5 (link) Some of the western blot results were quantified by densitometric scans using ImageJ (version 1.48p; W Rasband, National Institutes of Health; available at http://rsb.info.nih.gov/ij/ ) for the following Pearson’s correlation analysis. The primary antibodies of TEAD1 (sc-376113), TEAD4 (sc-134071), CTGF (L-20) (sc-14939) and Cyr-61 (sc-374129) were commercially available from Santa Cruz (Dallas, TX, USA). YAP1 (ab52771) antibody was achieved from Abcam (Cambridge, MA, USA). TEAD2 (SAB2102402) and TEAD3 (AV38278) antibodies were obtained from Sigma-Aldrich (St Louis, MO, USA). Other primary antibodies were from Cell Signaling (Danvers, MA, USA), including p21 (#2946), p27 (#2552), p-Rb (Ser807/811) (#9308), cleaved-caspase 3 (Asp175) (#9661), cleaved-caspase 7 (Asp198) (#9491), cleaved-PARP (Asp214) (#9541), cyclin D1 (#2978), cyclin D3 (#2936), CDK4 (#12790), CDK6 (#3136), c-Myc (#9402) and GAPDH (#2118). Anti-Mouse IgG-HRP (Dako, Glostrup, Denmark, 00049039, 1:30 000) and anti-Rabbit IgG-HRP (Dako, 00028856, 1:10 000) were used for secondary antibodies.
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