2 2 diphenyl 1 picrylhydrazyl (dpph)

Manufactured by Yuanye Bio-Technology

Sourced in China

DPPH is a stable free radical that is commonly used in antioxidant assays. It absorbs light at 517 nm, and its absorbance decreases when it is reduced by an antioxidant compound.

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Lab products found in correlation

35 protocols using 2 2 diphenyl 1 picrylhydrazyl (dpph)

Antioxidant Activity Evaluation of AL

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The AL was diluted with anhydrous ethanol to a concentration between 0.7% to 50%. Following this, we added it to a 96-well plate (Sample group: 100 μL AL sample + 100 μL DPPH (Yuanye Biotechnology Co., Ltd., Shanghai, China); Blank group: 100 μL anhydrous ethanol + 100 μL DPPH), then mixed thoroughly, kept the mixture in the dark for 30 min, and measured the absorbance at 517 nm using an enzyme marker. Vitamin C (Vc) was employed as the positive control, while the blank group consisted of anhydrous ethanol in place of the AL sample. We then calculated SA (%) according to the following expression and subsequently calculated the EC₅₀ value of AL.

A_Samples (or A_blanks) are DPPH treated with AL (or ethanol absolute) at 517 nm.

Wang Z., Wang Q., Zhong W., Liang F., Guo Y., Wang Y, & Wang Z. (2023). Moisturizing and Antioxidant Effects of Artemisia argyi Essence Liquid in HaCaT Keratinocytes. International Journal of Molecular Sciences, 24(7), 6809.

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DPPH Radical Scavenging Activity Assay

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The method of DPPH radical scavenging activity was measured as suggested by previous reports with appropriate modifications [46 (link)]. DPPH (1,1-diphenyl-2-picrylhydrazyl) (Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China) reserve solution of 0.15 mmol/L was prepared and diluted with anhydrous ethanol to obtain DPPH working solution. A sample solution at a volume of 2 mL was added to 2 mL of DPPH working solution. The mixture was placed in the dark for 30 min and the absorbance was measured by a UV-1800 VIS spectrophotometer (Shanghai Meipuda instrument Co., Ltd., Shanghai, China) at 517 nm. The clearance rate was calculated as follows:

Clearance rate % = (1 - \frac{A_{1} {- A}_{2}}{A_{0}}) \times 100 %

where A₁ is the absorbance of the mixture of 2 mL sample solution and 2 mL DPPH, A₂ is the absorbance of the mixture of 2 mL sample solution and 2 mL ethanol, and A₀ is the absorbance of the mixture of 2 mL DPPH and 2 mL ethanol.

Zhang M., Ma W., Wang C., Yang X., Lou Y., Xia X, & Xu H. (2021). Optimization of Enzyme-Assisted Extraction and Purification of Flavonoids from Pinus koraiensis Nut-Coated Film and Antioxidant Activity Evaluation. Molecules, 26(7), 1950.

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Antioxidant-Rich Peach Coating Development

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Yellow peaches were obtained from Baoding Industry Group Co., Ltd. (Baoding, Hebei, China). Sodium alginate and glycerol were all purchased from Wanke Chemical Reagent Co., Ltd. (Baoding, Hebei, China). Folinphenol, gallic acid and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai China). Soybean oil was purchased from a supermarket in Baoding. The main reagents were listed.

Lu X., Chen Z., Ma Q., Mu J., Li X, & Liu H. (2022). Preparation and Characterization of Yellow Peach Peel/Sodium Alginate/Glycerol Antioxidant Film Applicable for Oil Package. Polymers, 14(9), 1693.

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Chicken Liver Antioxidant Assays

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Chicken liver was obtained from a commercial slaughterhouse (Jiangsu Lihua Co., Ltd, Changzhou, China), which was placed in crushed ice packaged in vacuum bags and then quickly transported to the laboratory at −18°C for storage. D-xylose, sucrose, D-galactose, D-fructose, glucose, and maltose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Pancreatin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferrozine mono-sodium salt, o-phthaldialdehyde (OPA), and L-carnosine were supplied by Shanghai Yuanye Biotechnology Co., Ltd. (Songjiang District, Shanghai, China). All other reagents used were of analytical grade.

Xiong G.Y., Chen X., Zhang X.X., Miao Y., Zou Y., Wang D.Y, & Xu W.M. (2020). Process optimization and the relationship between the reaction degree and the antioxidant activity of Maillard reaction products of chicken liver protein hydrolysates. Poultry Science, 99(7), 3733-3741.

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Comprehensive Analytical Reagent Specification

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The water used in this study was deionized (18 MΩ·cm) from Water Purification System. Sephadex G-100, DEAE-52, 2,2-diphenyl-1-picrylhydrazyl, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid, 2,4,6-tri(2-pyridyl)-s-triazine, 6-hydroxy-2,5,7,8-tetramethlchroman-2-carboxylic acid, casein, glacial acetic acid, Tris-base, alcalase, trypsin, papain, neutrase, pepsin, and ammonium persulfate were purchased from Yuanye Biotech Co. Shanghai, China. Absolute ethanol (analytical grade), acetic acid, acetone, ferric chloride, ferrous sulphate, glucose, methanol (analytical grade), perchloric acid, phenol (redistilled reagent grade), potassium per-sulphate, potassium sulphate, sodium acetate, and sodium hydroxide were purchased from DaMao Chemical Co. Tianjin, China. Boric acid, copper sulfate, hydrochloric acid, methylene blue indicator, methyl red indicator, petroleum ether, sulfuric acid, and sodium thiosulfate were purchased from Guangzhou Chemical Co. (Guangzhou, China). Bovine serum albumin, Tris-HCl buffer, SDS, tetramethylethylenediamine were obtained from Sigma Chemical Co. (St. Louis, MO, USA) RIPA lysis buffer, acrylamide/bis-acrylamide mix, ladder protein, were purchased from Thermo Fisher Scientific Co. (Shanghai, China). All other chemicals were of analytical grade.

Zhang W., Wang Z., Ganesan K., Yuan Y, & Xu B. (2022). Antioxidant Activities of Aqueous Extracts and Protein Hydrolysates from Marine Worm Hechong (Tylorrhynchus heterochaeta). Foods, 11(13), 1837.

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Moso Bamboo Powder Antioxidant Properties

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Moso bamboo powder was collected from Chengdu Shuxiang Co., Ltd. (Chengdu, China). Potato starch and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). The spectra/Por® 2 standard RC dry dialysis tube (8–14 kDa) was purchased from Spectrum Laboratories, Inc. (Compton, CA, USA). Choline chloride was purchased from Sigma-Aldrich. (Burlington, MA, USA) Urea, dimethyl sulfoxide (DMSO), HCl solution, KOH solution, and other reagents or solvents were purchased from Kelong Chemical Reagent Co., Ltd. (Chengdu, China).

Sun X., Li Q., Wu H., Zhou Z., Feng S., Deng P., Zou H., Tian D, & Lu C. (2023). Sustainable Starch/Lignin Nanoparticle Composites Biofilms for Food Packaging Applications. Polymers, 15(8), 1959.

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Reagents and Media for Biochemical and Microbiological Assays

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Folin–Ciocalteu reagent (FCR) was purchased from Feijing Biotechnology Co., Ltd. (Fuzhou, China). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,6-dichloro-phenol-indophenol were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Plate count agar, potato dextrose agar, lauryl sulfate (lauryl tryptose) broth, and brilliant green bile lactose broth were purchased from Aoboxing Biological Technology Co., Ltd. (Beijing, China). Other chemicals were obtained from Kelong Chemical Reagent Factory (Chengdu, China).

Feng X., Zhou Z., Wang X., Bi X., Ma Y, & Xing Y. (2020). Comparison of High Hydrostatic Pressure, Ultrasound, and Heat Treatments on the Quality of Strawberry–Apple–Lemon Juice Blend. Foods, 9(2), 218.

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Characterization of Fungal Polysaccharides

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PE was purchased from Xi’an Chengxin Edible Fungus Planting Co., Ltd., Xi’an, China. DEAE cellulose, common dialysis bag (8–14 KDa), 2,2-diphenyl-1-picrylhydrazyl (DPPH, purity ≥ 98%), 4-nitrophenyl-d-pyridyl Glucosinolate (pNPG, purity ≥ 98%), and α-glucosidase(enzymatic activity ≥ 50 units/mg protein) were purchased from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China. Glucose, xylose, arabinose, mannose, rhamnose, galactose, glucuronic acid, and standard were purchased from Hefei Bomei Biological Technology Co., Ltd., Hefei, China. Other chemicals used in the experiment were of analytical grade.

Gong P., Long H., Guo Y., Wang S., Chen F, & Chen X. (2022). Isolation, Structural Characterization, and Hypoglycemic Activities In Vitro of Polysaccharides from Pleurotus eryngii. Molecules, 27(20), 7140.

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Bioactive Compounds Extraction from Poria cocos

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The Poria cocos sclerotia was collected from Jinzhai County, LuAn City, Anhui Province, China. Ferric chloride (FeCl₃), trisodium citrate dihydrate (C₆H₅Na₃O₇), ammonium iron (II) sulfate [Fe(NH₄)₂·(SO₄)₂·6H₂O], potassium hexacyanoferrate (II) (K₄ [Fe(CN)₆]), potassium thiocyanate (KSCN) and o-phenanthroline (C₁₂H₈N₂) were purchased from Tianjin Guangfu Technology Development Co., Ltd. Pepsin, trypsin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-ethylbenzothiazoline sulphonic acid-6 ammonium salt (ABTS) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Niferex capsule (150 mg/capsule) was manufactured by Kermers Urban Pharmaceuticals Inc, USA., Malondialdehyde (MDA) test kit was obtained from Shanghai Yaji Biotechnology Co., Ltd. AOAC low-iron diets (iron content≤ 2.3 mg kg⁻¹) were gained from Beijing Botai Hongda Biotechnology Co., Ltd. The water used in the experiment was ultrapure water, and the reagents were analytical alcohols.

Zhang Y., Huang J., Sun M., Duan Y., Wang L., Yu N., Peng D., Chen W, & Wang Y. (2023). Preparation, characterization, antioxidant and antianemia activities of Poria cocos polysaccharide iron (III) complex. Heliyon, 9(1), e12819.

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LERW Antioxidant and Enzyme Inhibition

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LERW was purchased from Youluhuan Ecological Agriculture Co., Ltd. (Bozhou, China). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azobis-3-ethylbenzothiazoline-6-sulfonate (ABTS), α-glucosidase, tannins, acarbose, and rutin were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). p-nitrophenyl-β-D-galactopyranoside (pNPG) was obtained from Alfa Aesar Chemical Co., Ltd. (Shanghai, China). Astilbin with 98% purity was purchased from Priva Technology Development Co., Ltd. (Chengdu, China). Liposomes were prepared by our lab with the size of 131.84 ± 0.67 nm [7 ].

Guo X., Zhou T., Xing H., Zhang Y., Fang J., Kang T., Yao C., Yan J., Huang Y, & Yao Q. (2023). Antioxidant and In Vivo Hypoglycemic Activities of Ethanol Extract from the Leaves of Engelhardia roxburghiana Wall, a Comparative Study of the Extract and Astilbin. Foods, 12(5), 927.

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