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Electromax dh10b t1 phage resistant competent cells

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The ElectroMax DH10B T1 Phage-Resistant Competent Cells are a strain of E. coli bacteria that are designed for efficient transformation of DNA via electroporation. They are resistant to T1 phage infection, which can be a common issue in bacterial transformation experiments.

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Lab products found in correlation

1 mm gap cuvette, by Bio-Rad (4 mentions) Micropulser, by Bio-Rad (4 mentions)

4 protocols using electromax dh10b t1 phage resistant competent cells

1

Bacterial Transformation via Electroporation

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Example 3

1.5-2 μl of ligation mixture was transformed into 20 μl of ElectroMax DH10B T1 Phage-Resistant Competent Cells (Invitrogen, Cat #12033-015) by electroporation using MICROPULSER™ (BioRad) set at 1.7 kV using a 1 mm gap cuvette (BioRad, Cat #165-2089); after a pulse 1 ml SOC was added to cells and cells were incubated at 37° C. for 1 h with shaking at 200 rpm. 10 μl of recovery mixture was plated on LB agar plates containing ampicillin at a concentration of 100 μg/ml. Plates were incubated overnight at 37° C.

US10689656B2. Expression constructs and methods of genetically engineering methylotrophic yeast (2020-06-23). Impossible Foods Inc. [US]. Inventors: Smita Shankar [US], Martin Andrew Hoyt [US].
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2

Bacterial Transformation via Electroporation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 3

1.5-2 μl of ligation mixture was transformed into 20 μl of ElectroMax DH10B T1 Phage-Resistant Competent Cells (Invitrogen, Cat #12033-015) by electroporation using MicroPulser (BioRad) set at 1.7 kV using a 1 mm gap cuvette (BioRad, Cat #165-2089); after a pulse 1 ml SOC was added to cells and cells were incubated at 37° C. for 1 h with shaking at 200 rpm. 10 μl of recovery mixture was plated on LB agar plates containing ampicillin at a concentration of 100 μg/ml. Plates were incubated overnight at 37° C.

US09938327B2. Expression constructs and methods of genetically engineering methylotrophic yeast (2018-04-10). Impossible Foods Inc. [US]. Inventors: Smita Shankar [US], Martin Andrew Hoyt [US].
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3

Bacterial Transformation via Electroporation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 3

1.5-2 μl of ligation mixture was transformed into 20 μl of ElectroMax DH10B T1 Phage-Resistant Competent Cells (Invitrogen, Cat #12033-015) by electroporation using MicroPulser (BioRad) set at 1.7 kV using a 1 mm gap cuvette (BioRad, Cat #165-2089); after a pulse 1 ml SOC was added to cells and cells were incubated at 37° C. for 1 h with shaking at 200 rpm. 10 μl of recovery mixture was plated on LB agar plates containing ampicillin at a concentration of 100 μg/ml. Plates were incubated overnight at 37° C.

US10273492B2. Expression constructs and methods of genetically engineering methylotrophic yeast (2019-04-30). Impossible Foods Inc. [US]. Inventors: Smita Shankar [US], Martin Andrew Hoyt [US].
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4

Bacterial Transformation via Electroporation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 3

1.5-2 μl of ligation mixture was transformed into 20 μl of ElectroMax DH10B T1 Phage-Resistant Competent Cells (Invitrogen, Cat #12033-015) by electroporation using MICROPULSER™ (BioRad) set at 1.7 kV using a 1 mm gap cuvette (BioRad, Cat #165-2089); after a pulse 1 ml SOC was added to cells and cells were incubated at 37° C. for 1 h with shaking at 200 rpm. 10 μl of recovery mixture was plated on LB agar plates containing ampicillin at a concentration of 100 μg/ml. Plates were incubated overnight at 37° C.

US11319544B2. Expression constructs and methods of genetically engineering methylotrophic yeast (2022-05-03). Impossible Foods Inc. [US]. Inventors: Smita Shankar [US], Martin Andrew Hoyt [US].
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