Mouse bone marrow cells were isolated from 4–6 week old male ICR mice (Nara Biotech, Seoul, Korea) by flushing the femurs and tibias with α-MEM. The cells were cultured overnight in α-MEM with 10% FBS and 1% antibiotic-antimycotic reagent and incubated in 5% CO2 incubator. Non-adherent cells were collected and seeded on adequate number of plates with M-CSF. After 3 days, non-adherent cells were washed out with fresh media, and the adherent cells were used as BMMs. M-CSF was treated at 30 ng/ml and RANKL was treated at 50 ng/ml concentration in α-MEM.
Icr mice
Manufactured by Nara Biotech
Sourced in United States
ICR mice are a laboratory mouse strain commonly used for scientific research. They are a genetically heterogeneous outbred stock, serving as a model for the study of various biological processes and disease conditions. The core function of ICR mice is to provide a genetically diverse population for experimental investigations.
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14 protocols using icr mice
1
Mouse Bone Marrow Macrophage Isolation
2
Sperm Exposure to Bisphenol A
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All animal procedures were conducted according to the guidelines and were approved by the Institutional Animal Care and Use Committee of Chung-Ang University, Seoul, Korea. Experimental mice were kept in a room with controlled temperature (22 ± 2°C), ventilation, and lighting (12-h light/dark cycles) and were provided ad libitum access to laboratory feed (Cargill Agripurina, Seongnam, Korea) and water. Spermatozoa were collected from sexually mature male ICR mice (Nara Biotech, Seoul, Korea) using a standard procedure25 (link)31 (link). Briefly, both cauda epididymides from each mouse were collected and the associated fat was removed. The sample was then placed on a piece of filter paper to remove any liquid. The cauda epididymides were placed in cell culture dishes with BM, and punctured using a sterile needle to facilitate the release of spermatozoa. After preincubation, the sperm suspension was incubated in BM (containing 4 mg/mL BSA) supplemented with 0.0001, 0.01, 1, and 100 μM of BPA for 6 h in air at 37°C. The control group consisted of DMSO-treated spermatozoa that did not contain BPA. For each of the independent experiments, three 3 male mice per replicate were considered.
3
Acclimating Female ICR Mice
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Six-week-old female ICR mice (20–25 g) were purchased from Nara Biotech (Korea). All animal procedures adhered to the guidelines of the Institutional Animal Care and Use Committee of Dankook University (approval #DKU-20-009). The mice were allowed to acclimatize for 1 week with ad libitum access to pellet feed and water at 23 ± 1°C and a humidity of 44 ± 2% and under a 12 h light and 12 h dark cycle.
4
Effects of Diet and Environment on Mice
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Sixty-five female ICR mice (aged 6 weeks) were purchased from Nara Biotech (Seoul, Korea) and were used following a 1-week quarantine and acclimatization period. Four animals per cage were housed in a room maintained at a temperature of 23±3°C with a relative humidity of 50±10%. The animals were provided tap water and commercial rodent chow (NIH-31 Open Formula Auto, Ziegler Bros Inc., Gardners, PA, USA) ad libitum. The procedures used in the animal experiment were approved by the Institutional Animal Care and Use Committee, Konkuk University (Seoul, Korea).
5
Balb/c Nude and ICR Mice
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Six-week-old female BALB/c nude mice and 8-week-old female ICR mice were purchased from Nara Biotech (Seoul, Korea). All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Yonsei University, Mirae Campus (YWCI-201706-011-03) and performed in accordance with the school guidelines and regulations.
6
Sperm Capacitation with Angiopoietin-like 4
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One day before the start of the experiment, BM was incubated with additional APN (20 ng/mL) at 37°C in an atmosphere of 5% CO2. Mouse spermatozoa were collected from the cauda epididymides of 12-week-old ICR mice (Nara Biotech, Seoul, Korea), as described by Lee et al. [19 (link)]. Briefly, the cauda epididymides were collected and transferred to cell culture dishes containing 2 mL of BM. After a 12-min preincubation, spermatozoa were incubated in BM media with 0.4% BSA for inducing capacitation [19 (link), 21 (link)] (with/without APN) for 90 min at 7°C in an atmosphere of 5% CO2. For each experiment, three male mice per replicate were used.
7
ICR Mice Housing and Experiments
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ICR mice [21 (link)] were obtained from Nara Biotech Co. (Pyeongteak, Korea). Their gender, age, and body weight were male, 6 weeks, and 25–30 g. The mice were housed in cages (five mice/cage) under specific pathogen-free conditions (21–24 °C and 40–60% relative humidity) with a 12 h light/dark cycle. In addition, they were given free access to standard rodent food (Orientbio Inc., Seongnam, Korea) and water. All animal experiments were approved by the Committee of Animal Care and Experiment of Chungnam National University (Daejeon, Korea) with a reference number (CNU-00973) and carried out following the guidelines of the Animal Care and Use Committee at Chungnam National University.
8
ICR Mouse Housing Conditions
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Four-week old male Institute of Cancer Research (ICR) mice were purchased from the Nara Biotech (Pyeongtaek, Korea). The mice were provided free access to a commercial rodent chow and tap water ad libritum, and housed under specific pathogen-free conditions with a 12 hours light/dark cycle at 22°C ± 2°C. All animal experimental procedures were conducted in compliance with the guidelines and regulations for the use and care of animals established by Yonsei University College of Dentistry (Seoul, Korea).
9
Acute Oral Toxicity of Corn Silk Extract
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To determine the acute toxicity of corn silk extract, a single oral dose was used, and the approximate lethal dose (LD) was determined. Twenty-four ICR mice (12 males with body weight of 22.7~23.8 g and 12 females with body weight of 18.3~19.5 g) aged 4 weeks were purchased from NARA Biotech (Seoul, Korea). Six mice of each gender were randomly assigned to the control or test group. For the test group, the administration dose was 2,000 mg/kg of corn extract (10 mL/kg). The individual dose was calculated based on body weight measurements of the day. The animals were given a single intragastric oral administration of corn extract using a disposable syringe (1 mL) equipped with a gastric tube.
Animals were fasted for approximately 3~4 h before administration. Mice were fed 2 h after administration. At 30 min, 1, 2, and 4 h after dosing, animals were monitored for clinical signs and mortality. They were then monitored at least once per day during the 14-d period. Their body weights were recorded before dosing and at 1, 3, 7, and 14 d after dosing. At the end of the observation period, all animals were sacrificed with CO2 gas. Complete gross postmortem analysis was then performed.
Animals were fasted for approximately 3~4 h before administration. Mice were fed 2 h after administration. At 30 min, 1, 2, and 4 h after dosing, animals were monitored for clinical signs and mortality. They were then monitored at least once per day during the 14-d period. Their body weights were recorded before dosing and at 1, 3, 7, and 14 d after dosing. At the end of the observation period, all animals were sacrificed with CO2 gas. Complete gross postmortem analysis was then performed.
10
Subacute Toxicity Study of Corn Silk Extract
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Twenty-four male ICR mice aged 4 weeks were purchased from NARA Biotech. For the subacute toxicity study, mice were randomly allocated to four groups based on the amount of corn silk extract supplements: control [0 mg corn silk extract/kg body weight (BW)], low dose (5 mg/kg BW), medium dose (50 mg/kg BW), and high dose (500 mg/kg BW). Corn silk extract was orally administered once daily for 4 weeks at a designated time frame. Dosing was performed using a disposable syringe equipped with a gastric tube. For the control group, the same excipient used for dissolution of the corn silk extract was administered.
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