The mouse cGAS-specific siRNA (#LQ-055608-01-0002) and human cGAS-specific siRNA (#L-015607-02-0005) were purchased from GE Dharmacon (USA). ISD Naked (#tlrl-isdn, 1 μg/μl stock), cGAMP (#tlrl-nacga23, 1 μg/μl stock), and Poly(I:C) (#tlrl-pic, 25 mM stock) were purchased from InvivoGen (Singapore) and dissolved in endotoxin-free water. Mouse IL-6 (#ab198572, 0.1 μg/ul stock) was purchased from Abcam (Singapore). The anti-human IL-6 neutralising antibody (#ab9324, 1 μg/ul stock) was purchased from Abcam (Singapore). The JAK/STAT3 pathway Inhibitor WP1066 (#sc-203282, 20 mM stock) was purchased from Santa Cruz Biotechnology (Singapore) and dissolved in DMSO (Sigma-Aldrich, Singapore). Cells were treated with 25 μM Poly(I:C), 7 μM WP1066, 2 μg/ml cGAMP, ISD or RNA:DNA hybrids for 4 h.
Mouse il 6
Manufactured by Abcam
Sourced in Singapore, United States
Mouse IL-6 is a recombinant protein that corresponds to the murine interleukin-6 (IL-6) cytokine. IL-6 is a pleiotropic cytokine involved in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Poly 1 c, by InvivoGen (1 mentions)
Isd naked, by InvivoGen (1 mentions)
Wp1066, by Santa Cruz Biotechnology (1 mentions)
Cgamp, by InvivoGen (1 mentions)
Mouse tnf α elisa kit, by Abcam (1 mentions)
Allegra x 30r, by Beckman Coulter (1 mentions)
3 protocols using mouse il 6
1
Modulating cGAS-mediated Immune Signaling
2
Renal Cytokine Quantification Protocol
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Renal tissue was weighed, added with 10 mL of tissue lysate (the ratio of the radio-immunoprecipitation assay [RIPA]: phenylmethylsulfonyl fluoride [PMSF] was 100:1), and ground at 4°C for 30 minutes by electronic tissue homogenizer (Shanghai Jingxin Industrial Development Co., Ltd., China). The tissue homogenate was obtained by centrifuging the suspension at 12,000 rpm for ten minutes (Beckman Coulter, USA, model: Allegra X-30R), followed by detecting the levels of IL-6, TNF-α, TNF-1R, and TNF-2R by corresponding ELISA Kit [Mouse IL-6 (ab100713), mouse TNF-α ELISA Kit (ab208348), mouse sTNF-1R ELISA kit (TNFRSF1A, ab202408), mouse sTNF-2R ELISA kit (TNFRSF1b, ab202412), Abcam, UK], respectively. All procedures were performed strictly according to the instructions.
3
Quantifying Steroid and Protein Levels
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The following enzyme immunoassays were used to quantify steroids and proteins in both cortical tissue samples and tissue culture lysates: AlloP and CORT (Arbor Assays), mouse IL-6 (Abcam), mouse 21-hydroxylase (Aviva Systems Biology), and 5-α-reductase (LifeSpan BioSciences). Plates were read at 450 nm wavelength using a SpectraMax M2 plate reader (Molecular Biosystems). Sample concentrations were determined using a standard curve of the standards provided in each kit. Steroid concentrations were quantified as picograms per milligram of tissue weight, and protein levels were quantified as picograms per milligram of total protein.
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