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Pharmingen transcription buffer set

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The BD PharMingen™ Transcription Buffer Set is a laboratory product designed for use in transcription experiments. It provides the necessary buffer solutions required for the in vitro transcription process. The set includes the specific components needed to facilitate the transcription of DNA into RNA, a fundamental step in gene expression studies.

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Lab products found in correlation

Attune nxt, by Thermo Fisher Scientific (6 mentions) Facscanto 2, by BD (2 mentions) Human fcr blocking reagent, by Miltenyi Biotec (2 mentions) Percp efluor 710, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

BD Pharmingen (37 citations) BD Pharmingen Transcription Factor Buffer Set (18 citations) Transcription Buffer Set (8 citations) BD Pharmingen Transcription Factor Phospho Buffer Set (4 citations) BD Pharmingen Transcription Factor Phospho (TFP) buffer set (2 citations)

3 protocols using pharmingen transcription buffer set

1

Immunophenotyping and Intracellular Staining

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Cells were washed in FACS wash buffer (PBS/2%FBS/0.1% NaN3) before Fc receptors were blocked using the Human FcR Blocking Reagent (Miltenyi Biotec) according to the manufacturer’s description. Cells were stained with anti-human monoclonal antibodies on ice before cells were washed twice in FACS wash and once in PBS/0.1% NaN3. In some experiments, cells were fixed using 2% paraformaldehyde/PBS (wt/vol). For detection of intracellular BLIMP1 and IRF4 expression, the BD PharMingen™ Transcription Buffer Set (BD Biosciences) was used. Flow cytometry analyses were performed using either FACS Canto II (BD Biosciences) or Attune NxT (ThermoFisher Scientific) flow cytometers and software analyses were performed using the FlowJo Cytometric software (TreeStar Inc.).
Szodoray P., Andersen T.K., Heinzelbecker J., Imbery J.F., Huszthy P.C., Stanford S.M., Bogen B., Landsverk O.B., Bottini N., Tveita A., Munthe L.A, & Nakken B. (2021). Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity. Cell reports, 36(6), 109525.
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2

Immunophenotyping and Intracellular Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in FACS wash buffer (PBS/2%FBS/0.1% NaN3) before Fc receptors were blocked using the Human FcR Blocking Reagent (Miltenyi Biotec) according to the manufacturer’s description. Cells were stained with anti-human monoclonal antibodies on ice before cells were washed twice in FACS wash and once in PBS/0.1% NaN3. In some experiments, cells were fixed using 2% paraformaldehyde/PBS (wt/vol). For detection of intracellular BLIMP1 and IRF4 expression, the BD PharMingen™ Transcription Buffer Set (BD Biosciences) was used. Flow cytometry analyses were performed using either FACS Canto II (BD Biosciences) or Attune NxT (ThermoFisher Scientific) flow cytometers and software analyses were performed using the FlowJo Cytometric software (TreeStar Inc.).
Szodoray P., Andersen T.K., Heinzelbecker J., Imbery J.F., Huszthy P.C., Stanford S.M., Bogen B., Landsverk O.B., Bottini N., Tveita A., Munthe L.A, & Nakken B. (2021). Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity. Cell reports, 36(6), 109525.
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3

Immunophenotyping of BLIMP1 and IRF4 in B Cells

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The cells were washed in 98% PBS/2% fetal calf serum (FCS) buffer. Next, the cells were stained with anti-human monoclonal antibodies on ice, then washed twice in the same buffer (98%PBS/2%FCS) and once in PBS. For detection of intracellular B-lymphocyte induced maturation protein 1 (BLIMP1) and interferon regulatory factor 4 (IRF4) expression, the BD Pharmingen Transcription Buffer Set (BD Biosciences; Franklin Lakes, NJ, USA; Cat# 562574) was used. BLIMP1 was detected using PE Rat monoclonal anti-human/mouse BLIMP1 (clone 6D3; BD Pharmingen; Franklin Lakes, NJ, USA; Cat# 564702). IRF4 was detected using Alexa Fluor 488 Rat monoclonal anti-human/mouse IRF4 (clone IRF4.3E4; Biolegends, San Diego, CA, USA; Cat# 646405). Finally, CD19-positive cells were gated using CD19 Monoclonal Antibody (SJ25C1), PerCP-eFluor™ 710, eBioscience™ (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA; Cat# 46-0198-42). Flow cytometry analyses were performed using Attune NxT (ThermoFisher Scientific; Waltham, MA, USA) flow cytometer, and FlowJo software was used to analyze the cells.
Upfold N.L., Petakh P., Kamyshnyi A, & Oksenych V. (2023). Tyrosine Kinase Inhibitors Target B Lymphocytes. Biomolecules, 13(3), 438.
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