Uv 1800 ultraviolet visible uv vis spectrophotometer

To evaluate the affinity to hydroxyapatite, CUR, CUR loaded HA-C₁₈ micelles and CUR loaded ALN-HA-C₁₈ micelles (2 mL, 0.5 mg/mL) were added into a 8 mL PBS solution separately. Then, 100 mg of hydroxyapatite was dispersed into the solution and gently shaken at room temperature. At the same time, CUR, CUR loaded HA-C₁₈ micelles and CUR loaded ALN-HA-C₁₈ micelles were respectively incubated at the same condition without hydroxyapatite as the control. After 1 h, the mixture solution was centrifuged (4000 rpm, 10 min) and the absorbance of the supernatant (diluted with the same volume methanol) was measured by a UV-1800 Ultraviolet-Visible (UV-Vis) spectrophotometer (Shimadzu, Tokyo, Japan) at 425 nm. The binding percentage with hydroxyapatite was calculated as the ratio of the difference between the control group and the test group to the control group.

The synthesis of AgNPs was characterized preliminarily by scanning the absorption maxima of the mixture at wavelengths between 300–700 nm on a UV-1800 ultraviolet-visible (UV-Vis) spectrophotometer (Shimadzu, Kyoto, Japan). The characterization of the synthesized AgNPs was conducted with a Rigaku X-ray diffractometer (Tokyo, Japan), operated at 2θ from 30° to 80° at 0.041°/minute with a time constant of 2 seconds. AgNP synthesis and the elemental composition were further confirmed by scanning electron microscopy–energy dispersive spectroscopy ([SEM–EDS] JEOL-64000; JEOL, Tokyo, Japan). The surface morphology, crystalline nature, and size of the synthesized AgNPs were examined using high-resolution transmission electron microscopy ([TEM] JEM-2010; JEOL) at an accelerating voltage of 200 kV. The spectra of the AgNPs were obtained by Fourier transform infrared spectroscopy ([FTIR] PerkinElmer, Waltham, MA, USA) in the diffuse reflectance mode at a resolution of 4 cm⁻¹ with KBr pellets.