Nk1.1buv661

Manufactured by BD

The NK1.1BUV661 is a lab equipment product. It is a fluorochrome-conjugated antibody used for the detection and analysis of natural killer (NK) cells in flow cytometry applications.

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2 protocols using nk1.1buv661

Multiparameter Flow Cytometry for Immune Cell Analysis

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Fluorochrome-conjugated antibodies were used to select and sort cell subsets, analyze T cell phenotypes, and to determine cytokine production. The following antibodies were used in this study, separated by manufacturer (clone is indicated in parentheses). Biolegend: Ki67-PacificBlue (16A8), CD62L-BV510 (MEL-14), CD73-BV605 (TY/11.8), CD44-FITC (IM7), PD1-PEDazzle594 (RMP1–30), TIGIT-PECy7 (1G9), LAG3-BV785 (C9B7W), IFNγ (XMG1.2), TNFα-PECy7 (MP6-XT22), SATB1-AlexaFluor594 (O96C6), TIM3-APC/Fire750 (B8.2C12), OX40-BV711 (OX-86), OX40L-PECy7 (RM134L), Tim3-PerCP/Cy5.5 (B8.2C12), CD8-FITC (53–6.7), CD90.2-PECy7 (30H12), CD90.2-PerCP/Cy5 (53–2.1), CD4-APCCy7 (RM4–5). || BD Biosciences: CD90.2-BUV395 (53–2.1), CD4-BUV496 (GK1.5), CD19-BUV661 (1D3), CD11c-BUV661 (N418), F4/80-BUV661 (T45–2342), NK1.1BUV661 (PK136), TER119-BUV661 (TER-119), CD127-BUV737 (SB/199), CD8-BUV805 (53–6.7), FR4-BV421 (12A5), CTLA4-APCR700 (UC10–4F10–11), NK1.1-eFluor450 (PK136), Ter-119-eFluor450 (Ter119), Rorγt-BV650 (Q31–378), CD62L-BV605 (MEL-14). || Invitrogen: FoxP3-AlexaFluor532 (FJK-16s), CD44-BUV737 (IM7), PD1-SB780 (J43), TOX-eFluor660 (TXRX10), EOMES-PerCP/eFluor710 (Dan11mag), F4/80-eFluor450 (BM8), CD49b-eFluor450 (DX5), CD11c-eFluor450 (N418), PD1-PerCPe710 (J43). || Santa Cruz Biotechnologies: NFATc1-AlexaFluor488 (7A6), CD30L-AlexaFluor680 (RM153).

Pollard J., Hynes G., Yin D., Mandal M., Gounari F., Alegre M.L, & Chong A. (2023). Pregnancy programs epigenetic and transcriptional exhaustion in memory CD8+ T cells. Research Square.

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Multiparameter Flow Cytometry Immune Profiling

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Single-cell-suspensions of splenocytes and liver leukocytes were stained for cell surface markers using specific fluorophore-conjugated antibodies optimized for flow cytometry. Reagents and antibodies used in this study were LIVE/DEAD Fixable Blue Dead Cell Stain (ThermoFisher), CD11b-BUV395 (Clone: M1/70; BD Biosciences), Siglec-F-BUV615 (Clone: E50-2440; BD Biosciences), NK1.1-BUV661 (Clone: PK136; BD Biosciences), B220-BUV737 (Clone: RA3-6B2; BD Biosciences), CD8α-BUV805 (Clone: 53–6.7; BD Biosciences), MHCII(I-A/I-E)-BV510 (Clone: M5/114.15.2; BioLegend), CD4-BV570 (Clone: RM4-5; BioLegend), SCA-1-BV711 (Clone: D7; BioLegend), CD11c-BV785 (Clone: N418; BioLegend), Ly6C-FITC (Clone: HK1.4; BioLegend), Siglec-H-PerCP/Cy5.5 (Clone: 551; BioLegend), F4/80-PE (Clone: BM8; BioLegend), CD3ε-PE/Cy5 (Clone: 145-2C11; ThermoFisher), CD80-PE/Cy7 (Clone: 16-10A1; BioLegend), CD115-AF594 (Clone: AFS98; BioLegend), CCR2-APC (Clone: 475301; R&D Systems), CD45-AF700 (Clone: 30-F11; BioLegend), CD48-APC/Cy7 (Clone: HM48-1; BioLegend), Ly6G-Biotin (Clone: 1A8; BioLegend; with secondary antibody streptavidin-DyLight 800; ThermoFisher). Stained and fixed (4% PFA; 10 min in the dark) cells were analyzed with a Becton Dickson custom 10-laser ‘LSR-II’ flow cytometer and FlowJo software (v.10.4.1).

Jung S.R., Ashhurst T.M., West P.K., Viengkhou B., King N.J., Campbell I.L, & Hofer M.J. (2020). Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection. PLoS Pathogens, 16(4), e1008525.

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