Interleukin 6 (il 6)

Manufactured by Selleck Chemicals

Sourced in United States

IL-6 is a lab equipment product that functions as an interleukin-6 protein. Interleukin-6 is a cytokine involved in various biological processes.

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Lab products found in correlation

Lenalidomide, by Selleck Chemicals (1 mentions) Stattic, by Selleck Chemicals (1 mentions) Interleukin 6 (il 6), by Immunotools (1 mentions) Um171, by Selleck Chemicals (1 mentions) Sfemii, by STEMCELL (1 mentions) Sb225002, by Selleck Chemicals (1 mentions) Sc144, by Selleck Chemicals (1 mentions) Gsk2982772, by Selleck Chemicals (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Viper, by Novus Biologicals (1 mentions) Lipopolysaccharides (lps), by Novus Biologicals (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Tnf α, by Selleck Chemicals (1 mentions) Recombinant human il 6, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Arpe 19, by American Type Culture Collection (1 mentions) Interleukin 6 (il 6), by Cell Signaling Technology (1 mentions)

5 protocols using interleukin 6 (il 6)

Inhibition of STAT3 by Stattic

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2 × 10⁶ cells were cultured in 2 ml of favorite medium, stimulated with 10 ng of IL-6 (ImmunoTools, Germany) and samples were collected at different time points. Cell lines that are usually cultured in medium supplemented with IL-6 were starved from this cytokine for 12 h previous to stimulus.
Stattic (Selleckchem, USA) was employed for 5 h at different doses to inhibit STAT3 and then IL-6 was added or not to the cells for 1 h. Cells were starved of IL-6 only during the 6 h of treatment.
Lenalidomide (Selleckchem, USA) was used at different doses for 4 or 24 h.

Canovas Nunes S., Manzoni M., Pizzi M., Mandato E., Carrino M., Quotti Tubi L., Zambello R., Adami F., Visentin A., Barilà G., Trentin L., Manni S., Neri A., Semenzato G, & Piazza F. (2018). The small GTPase RhoU lays downstream of JAK/STAT signaling and mediates cell migration in multiple myeloma. Blood Cancer Journal, 8(2), 20.

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Generating MDSC-like cells from CD34+

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Donor-derived CD34+ cells(kind gift from K. Rajewsky, MDC Berlin, Germany) were propagated in SFEM II(StemCell Technologies, 09605), SCF, FLT3-L, TPO, IL6(all 100ng/ml; easyexperiments.com), UM171(Selleck, 35nM), SR1(Selleck, 0.75μM), 19-deoxy-9-methylene-16,16-dimethyl PGE2(Cayman, 10μM). CD34+ differentiation towards immature MDSC-like cells was induced by switching culture medium to RHB-A-medium supplemented with human SCF(50ng/ml) and human GM-CSF(100ng/ml) for 7-12 days(cl.#1, cl.#2), prior to co-culture.

Schmitt M.J., Dramaretska Y., Barozzi I., Göhrig A., Kertalli S., Großmann M., Naumann H., Sanchez-Bailon M.P., Hulsman D., Glass R., Squatrito M., Serresi M, & Gargiulo G. (2020). Phenotypic mapping of pathological crosstalk between glioblastoma and innate immune cells by synthetic genetic tracing. Cancer discovery, 11(3), 754-777.

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Inflammatory Factors Modulate MUC16 Expression

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A total of 1x10⁶ HEY cells were first treated with 10, 50 and 100 ng/ml LPS (Sigma-Aldrich; Merk KGaA), IL-6 (PeproTech, Inc.) or IL-8 (PeproTech, Inc.), or 2.5, 10 and 25 ng/ml of TNF-α (PeproTech, Inc.) for 24, 48 and 72 h at 37˚C, with 1X PBS used as a control. The lowest concentration and shortest stimulation time of inflammation-associated factors resulting in a statistically significant change in MUC16 mRNA expression levels were selected as the optimal concentration and duration, respectively. HEY cells were treated with LPS, IL-6, IL-8 or TNF-α at the optimal concentration and for the optimal duration. For co-treatment,HEY cells were treated with 10 ng/ml LPS combined with 500 nM Toll-like receptor 4 (TLR4) antagonist VIPER (Novus Biologicals, LLC) for 48 h, 50 ng/ml IL-6 was combined with 10 µM membrane glycoprotein 130 (gp130) inhibitor SC144 (Selleck Chemicals) for 24 h, 50 ng/ml IL-8 was combined with 10 µM CXCR2 antagonist SB225002 (Selleck Chemicals) for 48 h or 2.5 ng/ml TNF-α was combined with 100 nM of its inhibitor GSK2982772 (Selleck Chemicals) for 24 h.

Liu J., Li L., Luo N., Liu Q., Liu L., Chen D., Cheng Z, & Xi X. (2020). Inflammatory signals induce MUC16 expression in ovarian cancer cells via NF-κB activation. Experimental and Therapeutic Medicine, 21(2), 163.

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Investigating STAT3 Activation and Regulation

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To examine the pattern of STAT3 activation, cells were treated with 50 ng/mL recombinant human IL6 (Peprotech, Cranbury, NJ) for 30 min. The medium containing IL6 was removed and the cells were washed with PBS. Fresh medium was added, and the cells were incubated at 37°C until the indicated times. To assess the effects of BZA (Selleck chemical, Houston, TX) on the activation of STAT3, cells were pretreated with the drug for 2 h prior to IL6 treatment. Cells were then cultured in the presence of BZA throughout the remainder of the experiment. To examine the effects of BZA on downstream IL6/STAT3 signaling, cells were plated at low density in 10 cm plates and treated with BZA for up to 72 h.

Wightman S.M., Alban T.J., Chen X., Lathia J.D., Wang Y, & Stark G.R. (2021). Bazedoxifene inhibits sustained STAT3 activation and increases survival in GBM. Translational Oncology, 14(11), 101192.

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ARPE-19 Cell Culture and IL-6 Treatment

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The human RPE cell line ARPE-19 was purchased from American Type Culture Collection (ATCC, Manassas, VA). The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Life Technologies, Grand Island, NY) containing 10% fetal bovine serum (FBS; Gibco) at 37 °C in a humidified atmosphere containing 5% CO₂. The cell line was authenticated with short tandem repeat (STR) analysis. The STR results showed that the DNA of the cell line perfectly matched ARPE-19, and no cross contamination of human cells was detected (Appendix 1).
For IL-6 treatment, the cells were seeded in six- or 96-well plates and incubated with different concentrations of IL-6 (Cell Signaling, Danvers, MA). The pharmacological inhibitor of STAT3 signaling S3I-201 was purchased from Selleck Chemicals (Houston, TX), prepared in dimethyl sulfoxide (DMSO) as 10 mM stock solution, and added 60 min before treatment with IL-6.

Chen X., Yang W., Deng X., Ye S, & Xiao W. (2020). Interleukin-6 promotes proliferative vitreoretinopathy by inducing epithelial-mesenchymal transition via the JAK1/STAT3 signaling pathway. Molecular Vision, 26, 517-529.

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