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Apc conjugated anti human cd206 antibody α hmrc1

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The APC)-conjugated anti-human CD206 antibody (α-hMRC1) is a laboratory reagent used for the detection and analysis of CD206, a cell surface receptor, in human samples. This antibody is conjugated to the fluorescent dye APC, allowing for flow cytometric detection and quantification of CD206-expressing cells.

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Lab products found in correlation

Facscalibur, by BD (2 mentions) Apc conjugated mouse igg1, by BD (2 mentions)

2 protocols using apc conjugated anti human cd206 antibody α hmrc1

1

Quantifying Cell-Surface hMRC1 Expression

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For analysis of cell-surface expression of hMRC1, cells were washed twice with ice-cold 20 mM EDTA-PBS, followed by two washes in ice-cold 1% BSA-PBS. Cells were stained in 1% BSA-PBS for 30 min at room temperature in the dark with allophycocyanin (APC)-conjugated anti-human CD206 antibody (α-hMRC1; BioLegend, San Diego, CA, USA) or APC-conjugated Mouse IgG1 (BD Biosciences Immunocytometry Systems, Mountain View, CA, USA) as isotype control. Cells were then washed twice with ice-cold 1% BSA-PBS and fixed with 1% formaldehyde in PBS. Finally, cells were analyzed on a FACSCalibur (BD Biosciences Immunocytometry Systems, Mountain View, CA, USA). Data analysis was performed using FlowJo (Tree Star, San Carlos, CA, USA).
Sukegawa S., Miyagi E., Bouamr F., Farkašová H, & Strebel K. (2018). Mannose Receptor 1 Restricts HIV Particle Release from Infected Macrophages. Cell reports, 22(3), 786-795.
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2

Quantifying Cell-Surface hMRC1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of cell-surface expression of hMRC1, cells were washed twice with ice-cold 20 mM EDTA-PBS, followed by two washes in ice-cold 1% BSA-PBS. Cells were stained in 1% BSA-PBS for 30 min at room temperature in the dark with allophycocyanin (APC)-conjugated anti-human CD206 antibody (α-hMRC1; BioLegend, San Diego, CA, USA) or APC-conjugated Mouse IgG1 (BD Biosciences Immunocytometry Systems, Mountain View, CA, USA) as isotype control. Cells were then washed twice with ice-cold 1% BSA-PBS and fixed with 1% formaldehyde in PBS. Finally, cells were analyzed on a FACSCalibur (BD Biosciences Immunocytometry Systems, Mountain View, CA, USA). Data analysis was performed using FlowJo (Tree Star, San Carlos, CA, USA).
Sukegawa S., Miyagi E., Bouamr F., Farkašová H, & Strebel K. (2018). Mannose Receptor 1 Restricts HIV Particle Release from Infected Macrophages. Cell reports, 22(3), 786-795.
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