The antibodies used were LDH (Santa Cruz, sc-133123), Histone H3 (Abcam, ab1791) Actinin (Santa Cruz, sc-17829), Streptavidin-HRP (Cell Signaling 3999S), eIF3A (Abcam, ab118357), eIF4A1 (Abcam, ab31217), FXR1 (Abcam, ab129089), PABP (Abcam, ab21060), eIF5A (Abcam, ab32443), eIF2α (Invitrogen, AH00802), eIF4G1 (Abcam, ab2609), P53 (Santa-Cruz, sc-126 X), eIF4A3 (Abcam, ab32485), eIF4E (Cell Signaling, 20675), RPS6 (Abcam, ab58350), eIF3D (Abcam, ab12442), S6K1 (Abcam, ab32529), S6K1pT389 (Abcam, ab2571), RPS6pS235 (Abcam, ab12864), KDM5A (Abcam, ab70892). KDM4A and β-Actin antibodies were described in (23 (link)). MCM7 antibody was described in (8 (link)). KDM4A immunoprecipitations were performed with KDM4A-P006.
Eif3a
Manufactured by Abcam
EIF3A is a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex. The eIF3 complex plays a crucial role in the initiation of protein synthesis by facilitating the binding of the 40S ribosomal subunit to the mRNA.
Automatically generated - may contain errors
Lab products found in correlation
Eif4a3, by Abcam (1 mentions)
Eif4a1, by Abcam (1 mentions)
Streptavidin hrp, by Cell Signaling Technology (1 mentions)
Eif5a, by Abcam (1 mentions)
Eif2α, by Thermo Fisher Scientific (1 mentions)
Kdm5a, by Abcam (1 mentions)
Eif4e, by Cell Signaling Technology (1 mentions)
Eif4g1, by Abcam (1 mentions)
Histone h3, by Abcam (1 mentions)
Hnrnpa1, by Merck Group (1 mentions)
Vectashield mounting medium, by Vector Laboratories (1 mentions)
2 protocols using eif3a
1
Antibody-based Protein Interaction Analysis
2
Immunofluorescence Assay of hnRNPA1 and eIF3a
Check if the same lab product or an alternative is used in the 5 most similar protocols
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4.5x10 5 cells were plated 24 hr. before performing IFA. Cells were fixed in 4% paraformaldehyde solution and incubated for 10 min. at 37'C, followed by methanol fixation for 10 min. The cells were block using 6% BSA for 1 hr. at room temperature, followed by incubation with primary antibodies for 2-3 hr. The cells were then washed 3 times in 1X PBS/0.05% Tween 20, then incubated with secondary antibodies followed by PBS/0.05% Tween washes. IFA was performed using hnRNPA1 (Sigma, R4528; 1:500 dilution), eIF3a (Abcam, 3411S; 1:500 dilution) and Alexa Fluor 488-or 568-labeled donkey anti-rabbit or goat anti-mouse secondary antibodies (1:1,500 dilution, Life Technologies, A10042, A11029), and mounted with DAPI-containing VECTASHIELD mounting medium (Vector Labs). The images were obtained at the CNR Biological Imaging Facility, University of California at Berkeley.
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