Sumatriptan succinate (sumatriptan), α-methylserotonin maleate (MS), 1-(3-chlorophenyl) biguanide hydrochloride (mCPBG), 5-methoxytryptamine (MT), WAY208466, LP12, 2’,3’-dideoxyadenosine
(ddAdo), 2-hydroxyestradiol (2-CE), KH7, 2,4-dithenoyl-1,2,5-oxadiazone n2-oxide (HC-056456, HC), U73122, U73343, D609, ET-18-OCH3, neomycin, spermine, and bisindolylmaleimide 1 (Bis-1) were
purchased from Merck KGaA (Darmstadt, Germany). TCB2, BW723C86, and MK212 were purchased from TOCRIS Bioscience (Bristol, UK). H-89, mibefradil (Mib), and NNC 55–0396 (NNC) were purchased
from Cayman Chemical (Ann Arbor, MI, USA). Anti 5-HT2A receptor antibody (SR-2A (A-4); sc-166775) and anti-5-HT4 receptor antibody (SR-4 (G-3); sc-376158) were
purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Bedford, MA, USA). The molecular weight marker
set was purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). EzWestLumi Plus® was purchased from ATTO Corporation (Tokyo, Japan). Xestospongin C, anti-mouse IgG antibody
conjugated peroxidase, bovine serum albumin (BSA), fraction V, and other reagent grade chemicals were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).
(ddAdo), 2-hydroxyestradiol (2-CE), KH7, 2,4-dithenoyl-1,2,5-oxadiazone n2-oxide (HC-056456, HC), U73122, U73343, D609, ET-18-OCH3, neomycin, spermine, and bisindolylmaleimide 1 (Bis-1) were
purchased from Merck KGaA (Darmstadt, Germany). TCB2, BW723C86, and MK212 were purchased from TOCRIS Bioscience (Bristol, UK). H-89, mibefradil (Mib), and NNC 55–0396 (NNC) were purchased
from Cayman Chemical (Ann Arbor, MI, USA). Anti 5-HT2A receptor antibody (SR-2A (A-4); sc-166775) and anti-5-HT4 receptor antibody (SR-4 (G-3); sc-376158) were
purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Bedford, MA, USA). The molecular weight marker
set was purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). EzWestLumi Plus® was purchased from ATTO Corporation (Tokyo, Japan). Xestospongin C, anti-mouse IgG antibody
conjugated peroxidase, bovine serum albumin (BSA), fraction V, and other reagent grade chemicals were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).