Von kossa staining
Von Kossa staining is a histochemical technique used to detect the presence of calcium deposits in biological samples. The staining process involves the treatment of the sample with silver nitrate, which reacts with the calcium to form silver phosphate or silver carbonate. These silver compounds are then reduced to metallic silver, resulting in a black or brown staining of the calcium-containing areas within the sample. This method is commonly used in the analysis of bone, cartilage, and other mineralized tissues.
Lab products found in correlation
11 protocols using von kossa staining
Quantifying Aortic Calcium Deposition
Multilineage Differentiation Evaluation
Adipogenic and Osteogenic Differentiation of BMSCs
For osteogenic differentiation, BMSCs were seeded at 5 × 103/cm2 on 12-well plates and cultured for 21 days in osteogenic induction medium containing 0.1 µmol/L dexamethasone, 10 mmol/L β-glycerol phosphate, and 200 µmol/L ascorbate-2-phosphate. Cells cultured in MEMα medium supplemented with 15% FBS were used as a negative control. Osteogenic differentiation was detected by von Kossa staining (Sigma Aldrich) according to the manufacturer instructions and by real-time PCR analysis of osteopontin and Runx2 gene expression.
Histological Analysis of Tissue Samples
Mineral Deposition Quantification in Chondrocytes
Multilineage Differentiation of PDLSCs
Characterizing Osteogenic Mineralization
Multilineage Differentiation of Stem Cells
Quantification of Osteoblast Mineralization
von Kossa Staining of Mineralized PBS Scaffolds
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