Neocuproin

The following reagents were used in this study: Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, gallic acid, catechin, trolox, iron(II)sulfate, tryptic soy broth, ketoconazole, streptomycin, phosphate-buffed saline (PBS), and iron(III)chloride were purchased from Sigma Aldrich (St. Louis, MO, USA); sodium-carbonate from FHI Zdravlje AD Leskovac (Serbia); sodium nitrite from Alkaloid Skopje (Skopje, Macedonia); aluminum chloride, and tri-chloroacetic acid from Kemika (Zagreb, Croatia); sodium hydroxide from NRK Inzenjering (Belgrade, Serbia); 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid)-ABTS from Roche Diagnostics GmbH (Penzberg, Germany); neocuproin from Acros Organics (Geel, Belgium); monosodium phosphate and disodium phosphate from Merck (Boston, MA, USA); cuprum chloride from Fluka (Buchs, Switzerland); ammonium acetate and ethanol from Zorka Pharma-Hemija (Šabac, Serbia); gentamicin from Panfarma (Belgrade, Serbia); crystal violet from Bio-Merieux (France); methanol and ferrous sulfate from Zorka Šabac, Serbia; high-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS); 2 mM L-glutamine from Thermo Fisher Scientific (Altrincham, UK); and penicillin as bought from Panfarma, Šabac, Serbia.

The following reagents were used: Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, gallic acid, catechin, Trolox, iron(II)sulfate, and iron(III)chloride were bought from Sigma Aldrich (USA), sodium-carbonate from Zdravlje (Serbia), sodium nitrite from Alkaloid Skopje (Macedonia), aluminum chloride and trichloroacetic acid were from Kemika (Croatia), sodium hydroxide from NRK Inzenjering (Serbia), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS was from Roche Diagnostics GmbH (Germany), neocuproin was from Acros Organics (Belgium), monosodium phosphate and disodium phosphate were from Merck (USA), cuprum chloride was from Fluka (Germany), and ammonium acetate and ethanol were from Zorka Pharma (Serbia).