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4 protocols using na93v

1

Chromatin Modification Antibody Validation

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H3K27ac (39,133, Active Motif Lot # 31814008), H3K27ac (39,685, Active Motif, no. 14517014), H3K18ac (ab1191, Abcam, no. GR3211480–1), H3K27me3 (9756, Cell Signaling), H3K9ac (ab4441, Abcam), H4K16ac (39,167, Active Motif), H3 general (ab1791, Abcam, no. GR177884–2), Spike-In antibody (61,686, Active Motif, Lot# 00419007), p300 (sc-584, Santa Cruz, Lot # F3016), Gapdh (2118, Cell Signaling, Lot # 10), CBP(D6C5) (7389S, Cell Signaling), p53(CM5) (NCL-L-p53-CM5p, Leica Biosystems), PKCs p2056 (ab18192, Abcam), KAP1/TRIM29 pS824 (A300-767A, Bethyl), Acetyl-p53 (Lys379) (2570, Cell Signaling), anti-mouse IgG-HRP (NA93V, GE, no.9773218), anti-rabbit IgG-HRP (170–6515, Bio-Rad, no. 350003248).
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2

Comprehensive Chromatin Immunoprecipitation Assay

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H3 general (ab1791, Abcam, Lot # GR177884–2), H3.3 (09–838, Millipore, Lot # 2578126), H3K4me1 (ab8895, Abcam, Lot # GR193882–2), H3K27ac (39133, Active Motif. Lot # 31814008), H3K27ac (39685, Active Motif, Lot # 14517014), H3K18ac (ab1191, Abcam, Lot # GR3211480–1), H3K64ac (ABE1057, Millipore, Lot # 2826017), H3K122ac (ab33309, Abcam, Lot # GR284790–3), Spike-In antibody (61686, Active Motif, Lot# 00419007), H3.1/2 (ABE154, Millipore), p300 (sc-584, Santa Cruz, Lot # F3016), Gapdh (2118, Cell Signaling, Lot # 10), Lamin A (ab26300, Abcam, Lot # GR155781–1), b-Tubulin (T5201, Sigma, Lot # 088K4832), H3S10ph (39253, Active Motif, Lot # 8308001), H3S28ph (07–145, Millipore, Lot # 27707), H3S31ph (ab92628, Abcam, Lot # GR296764–8), HIRA (WC15/WC119 hybridoma), DAXX (07–471, Millipore, Lot # 2841952), ATRX (ab97508, Abcam, Lot # GR267227–8), Oct4 (sc-5279, Santa Cruz, Lot # C3109), anti-mouse IgG-HRP (NA93V, GE, Lot # 9773218), anti-rabbit IgG-HRP (170–6515, Biorad, Lot # 350003248).
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3

Comprehensive Chromatin Immunoprecipitation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
H3 general (ab1791, Abcam, Lot # GR177884–2), H3.3 (09–838, Millipore, Lot # 2578126), H3K4me1 (ab8895, Abcam, Lot # GR193882–2), H3K27ac (39133, Active Motif. Lot # 31814008), H3K27ac (39685, Active Motif, Lot # 14517014), H3K18ac (ab1191, Abcam, Lot # GR3211480–1), H3K64ac (ABE1057, Millipore, Lot # 2826017), H3K122ac (ab33309, Abcam, Lot # GR284790–3), Spike-In antibody (61686, Active Motif, Lot# 00419007), H3.1/2 (ABE154, Millipore), p300 (sc-584, Santa Cruz, Lot # F3016), Gapdh (2118, Cell Signaling, Lot # 10), Lamin A (ab26300, Abcam, Lot # GR155781–1), b-Tubulin (T5201, Sigma, Lot # 088K4832), H3S10ph (39253, Active Motif, Lot # 8308001), H3S28ph (07–145, Millipore, Lot # 27707), H3S31ph (ab92628, Abcam, Lot # GR296764–8), HIRA (WC15/WC119 hybridoma), DAXX (07–471, Millipore, Lot # 2841952), ATRX (ab97508, Abcam, Lot # GR267227–8), Oct4 (sc-5279, Santa Cruz, Lot # C3109), anti-mouse IgG-HRP (NA93V, GE, Lot # 9773218), anti-rabbit IgG-HRP (170–6515, Biorad, Lot # 350003248).
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4

Evaluating Stem Cell Regulatory Factors

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KAP1 (ab10483, Abcam), OCT4 (sc-5279, Santa Cruz), H3 general (ab1791, Abcam), antimouse IgG-HRP (NA93V, GE, Lot # 9773218), anti-rabbit IgG-HRP (170-6515, Biorad, Lot # 350003248), H3K9me3 (ab8898, Abcam), H3K27ac (39133, Active Motif), KLF5 (61099, Active Motif), KLF9 (701888, Thermo Fisher).
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