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Celltiter glo luminescent cell viability assays kit

Manufactured by Promega
Sourced in United States

The CellTiter-Glo Luminescent Cell Viability Assays kit is a homogeneous method for determining the number of viable cells in culture. The assay quantifies the amount of ATP present, which signals the presence of metabolically active cells. The kit utilizes a luminescent readout to measure cell viability.

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2 protocols using celltiter glo luminescent cell viability assays kit

1

Cell Viability Luminescence Assay

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Cell death was analyzed using CellTiter-Glo Luminescent Cell Viability Assays kit (G7571, Promega, USA). The Luminescent Cell Viability Assays were performed according to the manufacturer’s instructions [8 (link)]. In brief, 1.0 × 105 cells were seeded in 96-well plates with white wall. After treatment, an equal volume of CellTiter-Glo reagent was added to the cell culture medium, which had been equilibrated to room temperature for 30 min. Cells were shaken at 220 rpm for 15 min at room temperature. Luminescent recording was performed with Spark 20 M microplate reader (Tecan, Switzerland).
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2

Cell Viability Assay in MKC Cells

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Cell viability was measured at 48 h after transfection in MKC cells with CellTiter-Glo Luminescent Cell Viability assays Kit (Promega; G7570) according to the manufacturer’s instructions.
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