Ha tag 51064 2 ap

Western blot assay was performed as detailed in the previous article [31 (link)]. Antibody details were as follows: VHL (#68547, Cell Signaling Technology), FBXO22 (13606-1-AP, Proteintech), HIF-1α (11587-1-AP, Proteintech), GAPDH (60004-1-AP, Proteintech), VEGF (19003-1-AP, Proteintech), Cyclin D1 (2978T, Cell Signaling Technology), Cyclin E2 (4132T, Cell Signaling Technology), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Ubiquitin (#3936, Cell Signaling Technology. All Western blot experiments were repeated a minimum of three times.

The STAT1 (9172), STAT2 (72604), phosphor-STAT1 (9649), phosphor-STAT2 (88410), and IRF9 (76684) antibodies were purchased from Cell Signaling Technology (Danvers, MA). β-Actin (66009-1-Ig), GFP-tag (66002-1-Ig), Flag-tag (20543-1-AP), and HA-tag (51064-2-AP) antibodies were purchased from Proteintech (Chicago, IL). Recombinant human IFN-α (CYT-204) was purchased from ProSpec (Ness Ziona, Israel). The jetPRIME® transfection reagent was purchased from Polyplus-transfection® SA (Illkirch, France). Streptavidin magnetic beads (HY-K0208) and protein A/G magnetic beads (HY-K0202) were purchased from MedChemExpress (Monmouth Junction, NJ).

Amyloid beta-peptide (1-42) (PA4391) was purchased from Ontores Biotechnologies (Zhejiang, China). Recombinant human Dectin-1 (rhDectin-1) was purchased from Sino Biological (10215-HNCH; Beijing, China). HA-tag (51064-2-AP) and Flag-tag (20543-1-AP) were ordered from Proteintech (Shanghai, China). Biotin (D8150) and Biotinylated-Aβ₄₂ (Bio-Aβ₄₂) was obtained from GL Biochem (Shanghai, China). Penicillin/Streptomycin and Opti-MEM were ordered from Gibco (Carlsbad, CA, USA). Annexin V-FITC/PI Apoptosis Detection Kit was purchased from BD Biosciences (San Diego, CA, USA). Bovine serum albumin (BSA), Dulbecco's modified Eagle's medium (DMEM) and Dimethyl sulfoxide (DMSO) were ordered from Sigma (St. Louis, MO, USA). MTT powder, reactive oxygen species assay kit (ROS), JC-1 assay kit, RIPA lysis buffer and Hoechst 33258 were bought from Beyotime Institute of Biotechnology (Shanghai, China). PVDF membrane, ECL Plus and Western Blot Marker were bought from Bio-Rad. The list of antibodies used and their source is presented in the supplementary informationTable S1. Sequence Information of β-Amyloid (1-42) used in this study was shown in the Supplementary InformationTable S2.