Isorhamnetin 3 o glucoside

The fresh PPL was collected from Hangzhou City (Zhejiang, China) in 01 September, 2020. These samples were identified by Professor Wang Ping from Zhejiang University of Technology. The voucher specimens (No. PPL 20200923) were deposited in Moganshan Campus of Zhejiang University of Technology. The leaves were air−dried naturally at room temperature, crushed, and passed through an 80−mesh sieve. After that, they were stored in a −18 °C freezer until use.
The standards of polyphyllin I (≥98%), II (≥98%), VI (≥98%), and VII (≥95%); dioscin (≥98%); kaempferol 3-O-gentiobioside (≥95%); isorhamnetin-3-O-glucoside (≥98%); and cisplatin were purchased from Shanghai Yuanye Biotechnology Co. Ltd., (Shanghai, China). Ultrapure water was produced by a Barnstead TII Pure Water System (Waltham, MA, USA). All other analytical−grade chemicals used in this experiment were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). A549 cells, MCF−7 cells, HepG2 cells, A431 cells, and HBE cells were purchased from Hunan Fenghui Biotechnology Co. Ltd. Roswell Park Memorial Institute (RPMI−1640). Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were obtained from Gbico (New York, NY, USA).

S-adenosyl-_L-methionine (SAM) was purchased from Shenggong (Shanghai, China). Gallic acid, EC, (−)-epigallocatechin, ECG, and EGCG were purchased from Sigma-Aldrich (St. Louis, MO, USA). ECG3″Me, ECG4″Me, EGCG3″Me, and EGCG4″Me, were purchased from Nagara Science Co., Ltd. (Gifu, Japan). Myricetin and tamarixetin were obtained from BioBioPha Co., Ltd (Kunming, China), and caffeic acid, ferulic acid, isoferulic acid, 3-O-MethylGallic acid, 4-O-MethylGallic acid, methyl gallate, methyl 4-O-methylgallate, cyanidin 3-O galactoside chloride, peonidin 3-O-galactoside chloride, isoquercitrin, isorhamnetin 3-O-glucoside and syringetin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Mearnsetin and laricitrin were from ChemFaces (Wuhan, China). Methyl 3-O-methylgallate was from MedChemExpress (Monmouth Junction, NJ, USA). Myricetin 3′, 4′-dimethyl ether was provided by Prof. Ai-Xia Chen of Shandong University, China.