The serum concentrations of estradiol (E2) were measured using the Ultra-Sensitive Estradiol RIA kit (DSL-4800) from Beckman Coulter (Brea, CA). The liver tissue levels of estrone (E1) and E2 were measured by a UPLC/MS-MS method with a Waters Acquity UPLC system connected with the Xevo TQ triple quadrupole mass spectrometer as we have previously described33 (link),34 (link). In brief, a Xevo-TQ triple quadruple mass spectrometer (Waters, Milford, MA, USA) recorded MS and MS/MS spectra using electro spray ionization (ESI) in positive ion (PI) and negative ion (NI) mode, capillary voltage of 3.0 kV, extractor cone voltage of 3 V, and detector voltage of 650 V. Cone gas flow was set at 50 L/h, and desolvation gas flow was maintained at 600 L/h. Source temperature and desolvation temperatures were set at 150 and 350 °C, respectively. Analytical separations of the mixture of 101 standards were conducted on the UPLC system using an Acquity UPLC HSS T3 1.8 μm 1 × 150 mm analytical column kept at 50 °C and at a flow rate of 0.15 mL/min.
Tq triple quadrupole mass spectrometer
Manufactured by Waters Corporation
The TQ triple quadrupole mass spectrometer is a high-performance analytical instrument designed for precise and accurate quantitative analysis. It features two quadrupole mass analyzers arranged in series, enabling the selective detection and quantification of target analytes with high sensitivity and specificity.
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Lab products found in correlation
Acquity uplc system, by Waters Corporation (2 mentions)
Ultra sensitive estradiol ria kit dsl 4800, by Beckman Coulter (2 mentions)
Xevo tq triple quadruple mass spectrometer, by Waters Corporation (2 mentions)
Hypersil gold c18 column, by Thermo Fisher Scientific (1 mentions)
Acquity uplc binary pump, by Waters Corporation (1 mentions)
3 protocols using tq triple quadrupole mass spectrometer
1
Quantification of Estrogen Levels using UPLC/MS-MS
2
Quantification of Estrogen Levels using UPLC/MS-MS
3
Triolein Quantification by UPLC-MS/MS
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Samples of medium and cell lysate were analyzed on a Xevo TQ triple quadrupole mass spectrometer equipped with an Acquity UPLC binary pump, column heater, and a 2777 autosampler (Waters, Milford, MA). Chromatographic separation was carried out using a 2.1 mm × 50 mm, 1.9 µm Hypersil GOLD C18 column (Thermo Scientific) maintained at 60 °C. A binary solvent system composed of 10 mM ammonium formate in 40% H 2 O:60% acetonitrile (v/v, eluent A) and 90% isopropyl alcohol:10% acetonitrile (v/v, eluent B) was used for the separations. The column was initially conditioned with 90% solvent A. Immediately following injection, the solvent composition was ramped to 95% solvent B over 2.5 min. At 2.6 min, the solvent composition was returned to 90% solvent A and held for 0.4 min before the next injection. The flow rate was held constant throughout the run at 400 µL/min. A total of 5 µL of the upper pentanol phase was directly injected from the plates by setting an appropriate depth for the autosampler syringe. SRM transitions for 12 C triolein and the 13 C 21 glyceryl trioleate internal standard were acquired as described above.
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