On the final day of the experiment, blood was collected into heparinized tubes via the cardiac puncture under CO2 anesthesia. Serum was separated using centrifuge (3000 g, 15 min) following blood clotting. The serum levels of aspartate transaminase (AST), and alanine transaminase (ALT) were determined by spectrophotometer using a commercially available GOT-GPT assay kit (Asan Pharmaceutical).
Got gpt assay kit
Manufactured by Asan Pharmaceutical
The GOT-GPT assay kit is a laboratory equipment used to measure the levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in biological samples. The kit provides a standardized method for the quantitative determination of these enzymes, which are important biomarkers for various medical conditions.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Annexin 5 fitc, by BD (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Silymarin, by Merck Group (2 mentions)
Hydroxyproline, by Merck Group (2 mentions)
P dimethylaminobenzaldehyde, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Chloramine t, by Merck Group (1 mentions)
Chloramines t, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid dtnb glutathione gsh, by Merck Group (1 mentions)
Pi apoptosis detection kit 1, by BD (1 mentions)
β nicotinamide adenine dinucleotide phosphate, by Merck Group (1 mentions)
5 protocols using got gpt assay kit
1
Cardiac Puncture for Serum Enzyme Assay
2
Serum Enzyme Levels Determination
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Blood was collected via the cardiac puncture under CO2 anaesthesia on the final day of the experiment. Serum was separated using centrifuge (3000 g, 15 min) following blood clotting. The serum levels of aspartate transaminase (AST), and alanine transaminase (ALT) were determined using a GOT-GPT assay kit (Asan Pharmaceutical, Korea).
3
Protective Effects of Phytochemicals
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TAA, silymarin, hydroxyproline, p-dimethylaminobenzaldehyde, 1,1,3,3-tetraethoexypropane, chloramine-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), and glutathione (GSH), standards of chlorogenic acid, p-coumaric acid, and rutin were purchased from Sigma (St. Louis, MO). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were acquired from Invitrogen (Carlsbad, CA). Perchloric acid was obtained from GFS Chemical Co. (Columbus, OH). GOT-GPT assay kit was purchased from Asan Pharmaceutical (Hwaseong-si, Korea). Annexin V-FITC and propidium iodide (PI) Apoptosis Detection Kit I was acquired from BD Biosciences (San Jose, CA). All other reagents used in this study were of the highest grade available commercially.
4
Silymarin Hepatoprotective Mechanism Investigation
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Silymarin, hydroxyproline, p-dimethylaminobenzaldehyde,1,1, 3,3-tetraethoxypropane (TEP), chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), glutathione (GSH), β-nicotinamide adenine dinucleotide phosphate, reduced form (β-NADPH), TAA and other reagents were purchased from Sigma, St. Louis, MO, USA. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were acquired from Invitrogen (Carlsbad, CA, USA). Perchloric acid was obtained from GFS Chemical Co. (Columbus, OH, USA). A GOT-GPT assay kit was purchased from Asan Pharmaceutical (Hwaseong-si, Korea). Annexin V-FITC and PI Apoptosis Detection Kit I were acquired from BD Biosciences (San Jose, CA, USA). All other reagents used in this study were of the highest grade available commercially.
5
Cardiac Puncture Blood Analysis
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On the final day of the experiment, blood samples were collected into heparinized tubes via cardiac puncture under CO2 anesthesia. Blood was immediately processed by centrifugation at 3,500 g for 15 min. Next, serum levels of aspartate transaminase (AST) and alanine transaminase (ALT) were measured by spectrophotometry using a commercially available GOT-GPT assay kit (Asan Pharmaceutical, Hwaseong-si, Korea).
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