Fluorochrome-labeled mAbs including mouse γδTCR (GL3, 1:100), Vγ4 (UC3-10A6, 1:500), Vγ1 (2.11, 1:500), CD24 (M1/69, 1:500), CD27 (LG.3A10, 1:500), CD62L (MEL-14, 1:500), and IL-17A (TC11-18H10.1, 1:500, Biolegend), CD44 (IM7, 1:200), RORγt (AFKJS-9, 1:200), and Ki-67 (SolA15, 1:200, eBioscience), IL-23R (753317, 1:10) and CCR6 (140706, 1:50, R&D system) were used. Anti-mouse Vγ6 (17D1, 1:500) was kindly provided by Dr. Tigelaar (Department of Dermatology, Yale University). For intracellular cytokine staining, cells were first blocked with anti-CD16/32 and then stained with different cell surface Abs. Cells were then fixed, permeabilized and stained intracellularly for IL-17, RORγt or Ki-67. The relevant isotype control mAbs were also used. Samples were harvested with BD FACS Calibur or Canto (Becton Dickinson, San Jose, CA) and analyzed with FlowJo software (TreeStar).
Il 23r 753317
Manufactured by R&D Systems
IL-23R (753317) is a recombinant human interleukin-23 receptor protein. It is a cell surface receptor that binds to interleukin-23, a cytokine involved in inflammatory and autoimmune responses.
Automatically generated - may contain errors
2 protocols using il 23r 753317
1
Multiparameter Flow Cytometry Analysis of γδ T Cells
2
Multiparameter Flow Cytometry Analysis of γδ T Cells
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Fluorochrome-labeled mAbs including mouse γδTCR (GL3, 1:100), Vγ4 (UC3-10A6, 1:500), Vγ1 (2.11, 1:500), CD24 (M1/69, 1:500), CD27 (LG.3A10, 1:500), CD62L (MEL-14, 1:500), and IL-17A (TC11-18H10.1, 1:500, Biolegend), CD44 (IM7, 1:200), RORγt (AFKJS-9, 1:200), and Ki-67 (SolA15, 1:200, eBioscience), IL-23R (753317, 1:10) and CCR6 (140706, 1:50, R&D system) were used. Anti-mouse Vγ6 (17D1, 1:500) was kindly provided by Dr. Tigelaar (Department of Dermatology, Yale University). For intracellular cytokine staining, cells were first blocked with anti-CD16/32 and then stained with different cell surface Abs. Cells were then fixed, permeabilized and stained intracellularly for IL-17, RORγt or Ki-67. The relevant isotype control mAbs were also used. Samples were harvested with BD FACS Calibur or Canto (Becton Dickinson, San Jose, CA) and analyzed with FlowJo software (TreeStar).
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