Complete blood count was performed as haematology diagnostics using ProCyte Dx Haematology Analyser (IDEXX, United States). Blood plasma haptoglobin and CRP concentrations were measured using Phase range Haptoglobin Assay Kit (cat. no. TP-801) and Phase porcine CRP Assay Kit (cat. no. TA-901) respectively (Tridelta Development Ltd., Ireland). Haptoglobin analyses were performed using an ADVIA 1800 Clinical Chemistry System autoanalyzer (Siemens Healthineers AG, Germany), while CRP concentrations were determined by solid-phase sandwich immunoassay, and measured using a POLARstar Galaxy microplate reader (BMG Labtech, Germany). The plasma concentration of cytokines (IFN-γ, IL-1β, IL-6, IL-10, IL-12, and TNF-α) were analyzed using methods previously described (33 (link)). Plasma diamine oxidase activity (DAO) was determined by a kinetic-fluorometric method where 1.5 diamino pentane (cadaverine, Sigma C8561) was the substrate and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) was the profluophore oxidized by the developed hydrogen peroxide. Units were defined as d-emission per min at 590 nm after excitation at 544 nm. Plasma lipopolysaccharide (LPS) concentration was analysed using the Porcine Endotoxin ELISA kit (AMSBIO, United Kingdom). The assays were performed in duplicates.
Procyte dx haematology analyser
Manufactured by IDEXX
Sourced in United States, France
The ProCyte Dx Haematology Analyser is a diagnostic instrument designed for in-vitro use in veterinary laboratories. It provides comprehensive analysis of blood samples, generating detailed information on various blood cell types and parameters. The ProCyte Dx utilizes advanced technology to deliver accurate and reliable results, supporting veterinary professionals in their clinical decision-making processes.
Automatically generated - may contain errors
Lab products found in correlation
11 protocols using procyte dx haematology analyser
1
Comprehensive Hematology and Inflammation Biomarkers
2
Comprehensive Blood Cell Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete blood cell counts (CBC) were determined from EDTA-treated blood samples using the clinical-grade Procyte Dx haematology analyser (IDEXX, Saint-Denis, France). Red blood cell parameters included red blood cell count (RBC), haematocrit (HCT), haemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), reticulocyte count (Ret) and immature reticulocyte fraction (IRF). CBC also included platelet count as well as white blood cell parameters: white blood cell count (WBC), neutrophil, lymphocyte and monocyte counts.
3
Blood Collection and Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anaesthetized with 3% isoflurane; approximately 0.7 mL of blood was collected by cardiac puncture and immediately decanted into EDTA blood tubes (Sarstedt AG & Co., Germany). Approximately 0.1 mL of blood was used for analyses of white blood cell counting by ProCyte Dx Haematology Analyser (IDEXX Laboratories, UK). Mice were then euthanized with CO2.
4
Complete Blood Cell Count Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete blood cell counts were determined from EDTA-treated blood samples using the clinical-grade Procyte Dx haematology analyser (IDEXX, Saint-Denis, France). White blood cell parameters included total white blood cell (WBC), neutrophil, eosinophil, lymphocyte and monocyte counts.
5
Longitudinal Hemogram Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Longitudinal blood collections for hemogram analyses were performed in mice under general anaesthesia with isoflurane; a puncture was performed with a 25 G needle in the mandibular vein, and the blood drop was collected to a capillary tube; the total amount of blood (~60 μL) was then dropped inside an EDTA tube and then temporarily stored at 4 °C; within a few hours the blood was gently transferred to an Eppendorf to be read in the ProCyte Dx Haematology Analyser (IDEXX, Westbrook, ME, USA). Hemogram analyses were planned for all genotype groups by 3, 6, 12, and 18 mo. In terminal blood collections, blood was collected to dry tubes, centrifuged at 2000× g-force for 10 min, and serum separated and stored at −80 °C. Exclusion criteria of hemogram data for analysis were all situations that could compromise leukocyte differential count as abnormal dot plots (see Supplementary File S1: Figure S3 ), presence of platelet aggregates, and platelets above 850 K/μL.
6
Monitoring Blood Safety After IBI Implantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To monitor the safety of IBI implantation, blood was collected before the baseline and before every cell injection: seven times for group 1 (six injections), four times for group 2 (three injections), and twice for group 3 (single injection). Complete blood count (CBC) parameters of whole blood, collected in EDTA anticoagulant, were measured in all beagles using an automated hematology analyzer (ProCyte DX® haematology analyser; IDEXX Laboratories, Inc., Westbrook, ME, USA).
7
Blood Biochemical Analysis using CatalystOne
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood biochemical analysis was performed using the CatalystOne® (Idexx, Westbrook, ME, USA) system. The blood samples for haematological and biochemical analysis were prepared according to standard protocols of the ProCyte Dx Haematology Analyser for blood cells and the Catalyst One (Idexx) System for biochemical analysis.
8
Cardiac Puncture for Blood Counting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At sacrifice, terminal blood samples were taken via cardiac puncture and transferred to EDTA-coated tubes. Blood counts were obtained using the ProCyte Dx Haematology Analyser (IDEXX).
9
Blood Collection and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
30 μl–50 μl of blood were collected in tubes containing EDTA and carefully mixed to avoid clotting. The ProCyte Dx haematology analyser (IDEXX, USA) was used for the generation of a complete haemogram.
10
Blood Sample Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples anti-coagulated with EDTA (1.8 mg/ml blood) or heparin, (both BD Biosciences, USA). All ProCyte DX were analysed using the IDEXX ProCyte DX Haematology analyser (IDEXX, USA). Results are expressed as absolute counts (K/µl), or ratios thereof.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!