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Sirna to aspm or prkcz encodingapkcζ

Manufactured by Thermo Fisher Scientific
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The SiRNA to ASPM or PRKCZ encoding aPKCζ is a laboratory equipment product designed for targeted gene silencing. It functions by delivering small interfering RNA (siRNA) molecules that specifically target the ASPM or PRKCZ genes, which encode proteins involved in cell division and signaling, respectively. This product allows for the knockdown of these genes in experimental settings, facilitating the study of their biological roles.

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Lab products found in correlation

Opti mem, by Thermo Fisher Scientific (4 mentions) Advanced dmem, by Thermo Fisher Scientific (2 mentions) Amniomax, by Thermo Fisher Scientific (2 mentions) Oligofectamine, by Thermo Fisher Scientific (2 mentions) Glutamax 1, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

SiRNAs

2 protocols using sirna to aspm or prkcz encodingapkcζ

1

Validation of ASPM and aPKCζ Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
H4 and Hela cells authenticated by short tandem repeat profiling were
obtained from ATCC, cultured in Advanced DMEM (ThermoFisher Scientific)
supplemented with 3% FBS (Altantis) and Glutamax-I (ThermoFisher
Scientific), and used within 5 passages with routine mycoplasma screening.
Ferret embryonic fibroblasts (FEFs) and mouse embryonic fibroblasts (MEFs) were
derived from post fertilization day 35 and 14.5 embryos, respectively. FEFs and
MEFs were cultured in AmnioMAX (ThermoFisher Scientific). Cells were transfected
with validated siRNA to ASPM or PRKCZ encoding
aPKCζ (ThermoFisher Scientific) using Oligofectamine and OptiMEM
(ThermoFisher Scientific) according to manufacturer’s instructions and
analyzed 48 h later.
Johnson M.B., Sun X., Kodani A., Borges-Monroy R., Girskis K.M., Ryu S.C., Wang P.P., Patel K., Gonzalez D.M., Woo Y.M., Yan Z., Liang B., Smith R.S., Chatterjee M., Coman D., Papademetris X., Staib L.H., Hyder F., Mandeville J.B., Grant P.E., Im K., Kwak H., Engelhardt J.F., Walsh C.A, & Bae B.I. (2018). Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature, 556(7701), 370-375.
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2

Validation of ASPM and aPKCζ Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
H4 and Hela cells authenticated by short tandem repeat profiling were
obtained from ATCC, cultured in Advanced DMEM (ThermoFisher Scientific)
supplemented with 3% FBS (Altantis) and Glutamax-I (ThermoFisher
Scientific), and used within 5 passages with routine mycoplasma screening.
Ferret embryonic fibroblasts (FEFs) and mouse embryonic fibroblasts (MEFs) were
derived from post fertilization day 35 and 14.5 embryos, respectively. FEFs and
MEFs were cultured in AmnioMAX (ThermoFisher Scientific). Cells were transfected
with validated siRNA to ASPM or PRKCZ encoding
aPKCζ (ThermoFisher Scientific) using Oligofectamine and OptiMEM
(ThermoFisher Scientific) according to manufacturer’s instructions and
analyzed 48 h later.
Johnson M.B., Sun X., Kodani A., Borges-Monroy R., Girskis K.M., Ryu S.C., Wang P.P., Patel K., Gonzalez D.M., Woo Y.M., Yan Z., Liang B., Smith R.S., Chatterjee M., Coman D., Papademetris X., Staib L.H., Hyder F., Mandeville J.B., Grant P.E., Im K., Kwak H., Engelhardt J.F., Walsh C.A, & Bae B.I. (2018). Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature, 556(7701), 370-375.
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