Sodium phosphate dihydrate

Manufactured by Merck Group

Sourced in Germany

Sodium phosphate dihydrate is an inorganic chemical compound with the formula Na2HPO4·2H2O. It is a white, crystalline solid that is soluble in water. The compound is commonly used as a laboratory reagent and in various industrial applications.

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Sodium phosphate (280 citations) Sodium dihydrogen phosphate dihydrate (65 citations) Sodium phosphate dibasic dihydrate (64 citations) Sodium phosphate monobasic dihydrate (54 citations) Sodium hydrogen phosphate dihydrate (8 citations) Sodium phosphate dibasic dihydrate (Na2HPO4·2H2O), (3 citations) EMPROVE® exp di-sodium hydrogen phosphate dihydrate (2 citations)

5 protocols using sodium phosphate dihydrate

Tryptic Digest of Reduced BSA

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D₂O (99.96 mol %
D) was acquired from Cambridge Isotopes (Andover, MA). Sodium phosphate
dihydrate, sodium phosphate monohydrate, sodium chloride, and formic
acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). Ethylene
glycol ReagentPlus (>99%) was purchased from Alfa Aesar (Ward Hill,
MA). The analytical sample used for these experiments was a tryptic
digest of fully reduced and iodoacetamide alkylated BSA (Thermo Scientific
Pierce BSA Protein Digest Standard, LC–MS grade, catalog no.
88341).

Anderson K.W, & Hudgens J.W. (2022). Chromatography at −30 °C for Reduced Back-Exchange, Reduced Carryover, and Improved Dynamic Range for Hydrogen–Deuterium Exchange Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 33(7), 1282-1292.

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Antioxidant Compounds Characterization

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Ethanol, sodium phosphate dihydrate, disodium dihydrogen phosphate, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), fluorescein 3’,6’-dihydroxyspiro[isobenzofuran-1(3H),9’-[9H]xanthen]-3-one and 2,20-azobis(2-methylpropionamidine) dihydrochloride (AAPH) were obtained from Sigma-Aldrich (Steinheim, Germany). Authentic standards of myricetin, caffeic acid, gallic acid, ferulic acid, protocatechuic acid, syringic acid, rosmarinic acid, chlorogenic acid, p-coumaric acid, and quercetin-3-glucoside were purchased from Sigma-Aldrich (St. Louis, MO, USA). Catechin, epigalloCatechin gallate, epiCatechin gallate, kaempferol-3-glucoside, rutin, apigenin, procyanidin B2, and luteolin were obtained from Extrasynthese (Genay, France).

Maleš I., Dobrinčić A., Zorić Z., Vladimir-Knežević S., Elez Garofulić I., Repajić M., Skroza D., Jerković I, & Dragović-Uzelac V. (2023). Phenolic, Headspace and Sensory Profile, and Antioxidant Capacity of Fruit Juice Enriched with Salvia officinalis L. and Thymus serpyllum L. Extract: A Potential for a Novel Herbal-Based Functional Beverages. Molecules, 28(9), 3656.

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Dissolution Study of Pharmaceutical Compounds

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Itraconazole, naproxen and phenytoin were obtained from Chemie Brunschwig (Basel, Switzerland). Cinnarizine, ketoconazole, probenecid, sodium chloride, sodium hydroxide and sodium phosphate dihydrate were obtained from Sigma-Aldrich (Steinheim, Germany). Dimethyl sulfoxide was obtained from VWR Chemicals (Søborg, Denmark). SIF powder was obtained from Biorelevant (South Croydon, UK). HPMCAS-L was gifted by Dow Wolf Cellulosics (Bomlitz, Germany) and PVPVA64 was obtained from BASF (Basel, Switzerland). All materials were used as received.

Blaabjerg L.I., Grohganz H., Lindenberg E., Löbmann K., Müllertz A, & Rades T. (2018). The Influence of Polymers on the Supersaturation Potential of Poor and Good Glass Formers. Pharmaceutics, 10(4), 164.

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Synthesis and Characterization of Iron Oxide Nanoparticles

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All solutions were prepared using reagents of high purity and ultra-pure water (Milli-Q, 18.2 MΩ cm⁻¹). Ferric chloride 98% (anhydrous FeCl₃), ferrous chloride tetrahydrate 98% (FeCl₂.4H₂O) and ammonium hydroxide 98% (NH₄OH) were purchased from Vetec. Phosphatidylcholine (PC) from egg yolk, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), phosphoethanolamine (PE) from egg yolk, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), cardiolipin (CL) from bovine heart and tetraoleoyl cardiolipin (TOCL) were acquired from Avanti Polar Lipids, Inc., Alabaster, AL. Cetyltrimethylammonium bromide (CTAB), sucrose, β-d-glucose, sodium phosphate dihydrate (NaH₂PO₄) and sodium phosphate monohydrate (Na₂HPO₄), and the redox mediator ferrocene were acquired from Sigma-Aldrich, St Louis, MO. Chloroform (P.A.) from LabSynth, Diadema, SP, Brazil.

Barreto G.R., Kawai C., Tofanello A., Neves A.A., Araujo-Chaves J.C., Belleti E., Lanfredi A.J., Crespilho F.N, & Nantes-Cardoso I.L. (2019). Magnetoliposomes as model for signal transmission. Royal Society Open Science, 6(1), 181108.

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Cerium Oxide Nanoparticle Synthesis

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Nano-pure diamond distilled water; 30% NH₄OH, 30% Hydrogen Peroxide, Cerium nitrate hexa-hydrate (trace metals basis, 99.999%), 1% Nafion, poly(acrylonitrile), sodium phosphate dihydrate, sodium diphosphate monohydrate, sodium acetate, hydrogen chloride, and sheep’s blood serum from Sigma Aldrich; Cerium Oxide nanoparticles 20% colloidal suspension in water from Alfa-Aesar; powder cerium oxide microparticles from REaction a John Mathis company.

Neal C.J., Gupta A., Barkam S., Saraf S., Das S., Cho H.J, & Seal S. (2017). Picomolar Detection of Hydrogen Peroxide using Enzyme-free Inorganic Nanoparticle-based Sensor. Scientific Reports, 7, 1324.

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