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Glycerol release

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The Glycerol release product is a laboratory equipment designed to measure the release of glycerol from biological samples. It provides a quantitative analysis of glycerol levels, which is a key indicator in various metabolic processes.

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Lab products found in correlation

Hexokinase activity assay, by Merck Group (2 mentions) Nonesterified fatty acids, by Abcam (2 mentions)

2 protocols using glycerol release

1

Assessing metabolic parameters in HFD mice

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Fasting serum glucose levels were measured by hexokinase activity assay (Sigma). Fasting insulin levels were assayed via ELISA (Crystal Chem). Serum nonesterified fatty acids (Abcam) and triglycerides (Eagle Diagnostics) levels were assayed according to manufacturers’ instructions. For lipolysis assays, adipocytes were isolated by collagenase treatment from epididymal adipose tissue of HFD-fed mice (15 (link)). Insulin-mediated inhibition of lipolysis in the presence of isoproterenol (0.1 μM) was assessed by glycerol release (Zen-Bio).
Kilroy G., Carter L.E., Newman S., Burk D.H., Manuel J., Möller A., Bowtell D.D., Mynatt R.L., Ghosh S, & Floyd Z.E. (2015). The Ubiquitin Ligase Siah2 Regulates Obesity-induced Adipose Tissue Inflammation. Obesity (Silver Spring, Md.), 23(11), 2223-2232.
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2

Assessing metabolic parameters in HFD mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fasting serum glucose levels were measured by hexokinase activity assay (Sigma). Fasting insulin levels were assayed via ELISA (Crystal Chem). Serum nonesterified fatty acids (Abcam) and triglycerides (Eagle Diagnostics) levels were assayed according to manufacturers’ instructions. For lipolysis assays, adipocytes were isolated by collagenase treatment from epididymal adipose tissue of HFD-fed mice (15 (link)). Insulin-mediated inhibition of lipolysis in the presence of isoproterenol (0.1 μM) was assessed by glycerol release (Zen-Bio).
Kilroy G., Carter L.E., Newman S., Burk D.H., Manuel J., Möller A., Bowtell D.D., Mynatt R.L., Ghosh S, & Floyd Z.E. (2015). The Ubiquitin Ligase Siah2 Regulates Obesity-induced Adipose Tissue Inflammation. Obesity (Silver Spring, Md.), 23(11), 2223-2232.
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