Two hundred and fifty nanograms of pGL3 reporter vector carrying the WT or mutant miR-148b-binding site (see plasmid construct, Figure 3 A), 25 ng of the phRL-SV40 control vector (Promega) and 100 nM miRNA precursors or scrambled sequence miRNA control (Ambion) were co-transfected into HEK293 cells in 24-well plates. Firefly luciferase activity was measured with a Dual Luciferase Assay Kit (Promega) 24 h after transfection and normalized with a Renilla luciferase reference plasmid.
Scrambled sequence mirna control
Manufactured by Thermo Fisher Scientific
The Scrambled sequence miRNA control is a laboratory reagent used as a control in miRNA experiments. It consists of a synthetic miRNA sequence that does not target any known miRNA in the organism being studied. This control can be used to assess the specificity and background of miRNA detection and quantification assays.
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Lab products found in correlation
2 protocols using scrambled sequence mirna control
1
Luciferase Assay for miR-148b Target
2
Dual-Luciferase Assay for miRNA Targeting
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Two hundred and fifty nanograms of pGL3 reporter vector carrying the WT or mutant miR‐425‐5p‐binding site (see plasmid construct, Fig. 4 D), 25 ng of the phRL‐SV40 control vector (Promega), and 100 nM miRNA precursors or scrambled sequence miRNA control (Ambion) were cotransfected into HEK293 cells in 24‐well plates. Firefly luciferase activity was measured with a Dual Luciferase Assay Kit (Promega) 24 hrs after transfection and normalized with a Renilla luciferase reference plasmid.
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