All reagents were of analytical grade. Methanol (MeOH), acetonitrile (ACN), and acetic acid (HAc) were acquired from Scharlau Chemie (Barcelona, Spain). Triethylamine (TEA) and 9-fluorenylmethoxycarbonyl (FMOC) chloride were obtained from Fluka (Buchs, Switzerland). Ammonium hydroxide (NH3·H2O), boric acid (H3BO3) and pentane were from Sigma (St. Louis, Missouri, USA), and ammonium acetate was from Merck (Darmstadt, Germany). 10,11-dihydroquinidine, 2,2’-azobisisobutyronitrile (AIBN), 3-(trimethoxysilyl)-propylmethacrylate (γ-MAPS), vinyltrimethoxysilane (VTMS), cetyltrimethylammonium bromide (CTAB), tetramethoxysilane (TMOS) and ethylene glycol (EG) were acquired from Aladdin Chemicals (Shanghai, China). dl -arginine, dl -histidine, dl -lysine, dl -serine, dl -threonine, dl -asparagine, dl -glutamine, dl -cysteine, dl -proline, dl -alanine, dl -valine, dl -leucine, dl -methionine, dl -phenylalanine, dl -tyrosine, d -tryptophan, l -tryptophan, dl -ornithine, dl -citrulline standards were from Fluka (Buchs, Switzerland), while dl -isoleucine, dl -carnitine, dl -aspartic acid, dl -glutamic acid, dl -norvaline, l -norvaline, dl -norleucine, dl -DOPA, dl -pyroglutamic acid and dl -methionine sulfone were obtained from Sigma (St. Louis, Missouri, USA). FMOC-amino acids were synthesized as reported previously [29 (link),30 (link)]. The dietary supplements were obtained in capsule form from online sources.
Dl norvaline
Manufactured by Merck Group
Sourced in Switzerland, United States
Dl-norvaline is a laboratory reagent used in various analytical procedures. It is a synthetic amino acid that serves as a precursor or intermediate in chemical reactions and assays. The product is offered in a pure form for use in research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Dl norleucine, by Merck Group (2 mentions)
L norvaline, by Merck Group (1 mentions)
Dl dopa, by Merck Group (1 mentions)
Dl isoleucine, by Merck Group (1 mentions)
Dl aspartic acid, by Merck Group (1 mentions)
Dl glutamic acid, by Merck Group (1 mentions)
Dl methionine sulfone, by Merck Group (1 mentions)
Pentane, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Uplc amino acid analysis solution, by Waters Corporation (1 mentions)
Dl methionine, by Merck Group (1 mentions)
Safe lock, by Eppendorf (1 mentions)
D methionine, by Merck Group (1 mentions)
D norvaline, by Merck Group (1 mentions)
Dl 2 aminobutyric acid, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Potassium sulfate k2so4, by Merck Group (1 mentions)
Dl alanine, by Merck Group (1 mentions)
5 protocols using dl norvaline
1
Amino Acid Standards and Dietary Supplements Analysis
2
Amino Acid Quantification in Fermentation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) was used to determine and quantify amino acids (Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val) in the fermentation supernatant. The AccQ-Tag reaction was performed according to the supplier’s instructions (UPLC Amino Acid Analysis Solution; Waters, MA, USA). Fermentation samples were diluted to the amino acid concentration range of the amino acid standard with three concentrations ranging from 50 to 400 nmol/mL. The amino acid DL-Norvaline (Sigma-Aldrich, MO, USA) was added to Amino Acid Standard Solution (5061-3330; Agilent, CA, USA) and the fermentation samples as an internal standard. The use of an internal standard with the calibration standard was used to correct volumetric errors introduced during sample preparation.
3
Synthesis and Characterization of Amino Acid Co-Crystals
Check if the same lab product or an alternative is used in the 5 most similar protocols
d -2-aminobutyric acid, d -norvaline, d -norleucine, l -2-aminoheptanoic acid, d -2-amino-octanoic acid, l -2-aminooctanoic acid, d -methionine, dl -2-aminobutyric acid, dl -norvaline, dl -norleucine, dl -2-aminoheptanoic acid, dl -2-amino-octanoic acid and dl -methionine were purchased from Sigma–Aldrich and used without further purification. The racemic amino acids mentioned here were recrystallized by solvent evaporation of an aqueous solution or hanging drop crystallisation as described by Smets et al. (2015 ▸ ), and subsequently measured using DSC. Liquid-assisted grinding was performed on a Retsch Mixer Mill MM 400 at 30 Hz to form co-crystals for the following quasi-racemates in a 1:1 molar ratio (100 mg in total); d -Abu:l -Nva, d -Abu:l -Hep, d -Nva:l -Hep, d -Nle:l -Hep, d -Met:l -Hep, d -Oct:l -Hep, d -Abu:l -Oct, d -Nva:l -Oct, d -Nle:l -Oct and d -Met:l -Oct. The samples were ground in a 2.0 ml Eppendorf SafeLock microcentrifuge tube with a 0.9 mm ball for 25 min using 10 µl ethanol, and a second time for 25 min after adding another 10 µl ethanol, and subsequently measured using DSC.
+ Expand
4
Preparation of Amino Acid Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glycine (E. Merck, INDIA), D,L-alanine (E. Merck, INDIA), D,L-nor-valine (>99.8%, Sigma Aldrich) and D,L-serine (>99.8 %, E. Merck, INDIA) were used after drying in vacuum desiccators at 370 K for 7 days. Potassium sulfate (K2SO4) of purity 99 % procured from E Merck, India. It was then dried in hot air oven at 500 K for 7 days and kept it for 3 days in vacuum desiccator prior to use. Triple distilled water (conductivity 0.6 μS/cm) was used in the entire study to prepare all aqueous solutions. Specifications of the compounds were summarized in Table 1 .
5
Mycobacterial Lipid Profiling via TLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wild-type and MceG strains were cultured in minimal media with 18 mM glycerol and 0.05% Tween80 in normal conditions. Low aeration conditions were performed by culturing the cells in 50 ml Falcon tubes with 45 ml of 7H9 medium that were parafilm-sealed and cultured at 37 ºC in static conditions. After 4 days of incubation, the cells were used to inoculate minimal media with 18 mM glycerol and normal aeration conditions. For the stringent response induction, DL-norvaline (0.5 mg ml -1 ) (Sigma) was added when the cells growing in minimal media with 18 mM glycerol reached an OD600 of 0.6. In each case, 50 ml aliquots were collected at different growth phases, washed with PBS and kept at -80 ºC. For the MAMEs extraction, frozen samples were lyophilized and 25 mg of dry weight were transferred to Pyrex glass tubes with PTFE caps (Supelco). MAMEs extraction was performed as previously described by Slayden and Barry, 2001 . TLC of MAMEs was performed on Silica Gel-60 plates (Meck) and developed a total of 5 times using petroleum ether/diethyl ether (95:5, v/v). Individual MAMEs were visualized by charring at 100 ºC using 5% molybdophosphoric acid in ethanol (Besra, 1998) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!