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Ptpn6fl fl

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Ptpn6fl/fl is a genetic mouse model that has the Ptpn6 gene flanked by loxP sites, allowing for conditional knockout or cell-specific deletion of the Ptpn6 gene. Ptpn6 encodes the protein tyrosine phosphatase, non-receptor type 6 (SHP-1), which plays a role in regulating immune cell signaling. This model can be used to investigate the function of SHP-1 in different cell types and physiological processes.

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Lab products found in correlation

Mb1 cre, by Jackson ImmunoResearch (2 mentions) Stat5fl fl, by Jackson ImmunoResearch (2 mentions) C57bl 6, by Jackson ImmunoResearch (2 mentions) Nod cg prkdcscid il2rgtm1wjl szj, by Jackson ImmunoResearch (2 mentions) Lyz2 cre, by Jackson ImmunoResearch (1 mentions) S100a8 cre, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

3 protocols using ptpn6fl fl

1

Genetic Manipulation of Mouse Immune Cells

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C57BL/6J mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained under specific pathogen–free conditions in the animal facilities at the University of Iowa. The mice were reared on a 12-hour light/dark cycle at a constant temperature of 22° ± 1°C. Ptpn6spin (MMRRC Repository, stock no. 015198-UCD) (7 (link)), Ptpn6fl/fl (Jackson Labs, stock no. 008336) (45 (link)),
Cd47−/− (Jackson Labs, stock no. 003173) (43 (link)), Lyz2-Cre (Jackson Labs, stock no. 004781) (46 (link)), and S100a8-Cre (Jackson Labs, stock no. 021614) (47 (link)) mice have been described previously.
Cd47−/− × Ptpn6spin DKO mice were generated by breeding
Cd47−/− and Ptpn6spin mice. Ptpn6Δmyeloid and Ptpn6ΔPMN mice were generated by breeding Ptpn6fl/fl mice with Lyz2-Cre and S100a8-Cre mice, respectively. All experimental procedures were approved by the Office of Animal Resources of the University of Iowa (Institutional Animal Care and Use Committee, protocol no. 0032004).
Mazgaeen L., Yorek M., Saini S., Vogel P., Meyerholz D.K., Kanneganti T.D, & Gurung P. (2023). CD47 halts Ptpn6-deficient neutrophils from provoking lethal inflammation. Science Advances, 9(1), eade3942.
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2

Generation and Use of Transgenic Mouse Models

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C57BL/6, NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ, Mb1-Cre, Stat5fl/fl, and Ptpn6fl/fl mice were purchased from The Jackson Laboratory. Mapk1fl/fl and Blnk−/− mice were obtained from Dr. Martin McMahon and Dr. Hassan Jumaa, respectively. MyceGFP/+ mice (Huang, C.Y. et al. 2008) were crossed with Bcl6mCherry/+ mice (Geng, H. et al. 2015) to generate MyceGFP/+; Bcl6mCherry/+ reporter mice. For animals bred in house, littermates of the same sex were randomized to experimental groups. All mouse breeding and experiments were subject to institutional approval by the Beckman Research Institute Animal Care and Use Committee. We have complied with all relevant ethical regulations. All mouse models used in the study are listed in Supplementary Table S7.
Chan L.N., Murakami M.A., Robinson M.E., Caeser R., Sadras T., Lee J., Cosgun K.N., Kume K., Khairnar V., Xiao G., Ahmed M.A., Aghania E., Deb G., Hurtz C., Shojaee S., Hong C., Pölönen P., Nix M.A., Chen Z., Chen C.W., Chen J., Vogt A., Heinäniemi M., Lohi O., Wiita A.P., Izraeli S., Geng H., Weinstock D.M, & Müschen M. (2020). Signaling input from divergent pathways subverts B-cell transformation. Nature, 583(7818), 845-851.
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3

Generation and Use of Transgenic Mouse Models

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C57BL/6, NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ, Mb1-Cre, Stat5fl/fl, and Ptpn6fl/fl mice were purchased from The Jackson Laboratory. Mapk1fl/fl and Blnk−/− mice were obtained from Dr. Martin McMahon and Dr. Hassan Jumaa, respectively. MyceGFP/+ mice (Huang, C.Y. et al. 2008) were crossed with Bcl6mCherry/+ mice (Geng, H. et al. 2015) to generate MyceGFP/+; Bcl6mCherry/+ reporter mice. For animals bred in house, littermates of the same sex were randomized to experimental groups. All mouse breeding and experiments were subject to institutional approval by the Beckman Research Institute Animal Care and Use Committee. We have complied with all relevant ethical regulations. All mouse models used in the study are listed in Supplementary Table S7.
Chan L.N., Murakami M.A., Robinson M.E., Caeser R., Sadras T., Lee J., Cosgun K.N., Kume K., Khairnar V., Xiao G., Ahmed M.A., Aghania E., Deb G., Hurtz C., Shojaee S., Hong C., Pölönen P., Nix M.A., Chen Z., Chen C.W., Chen J., Vogt A., Heinäniemi M., Lohi O., Wiita A.P., Izraeli S., Geng H., Weinstock D.M, & Müschen M. (2020). Signaling input from divergent pathways subverts B-cell transformation. Nature, 583(7818), 845-851.
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