All work with mice was conducted following institutional guidelines. Six-week-old female C57 BL/6 or C3H/HeJ mice (Jackson Laboratories, Bar Harbor, Maine) were used for assessing the colonization and the progress of infection of the respiratory tract. As required, 5–150 CFU of bacteria was delivered in 5 μL of PBS to the nares of mice anesthetized with isoflurane/oxygen. For the colonization profiles, groups of 4 mice were inoculated with WT or mutant bacteria, and at the indicated days 4 mice of each group were euthanized with carbon dioxide (CO2) and the nasal cavity, trachea, and lungs were collected in PBS and homogenized using a bead tissue disruptor. Bacterial load was enumerated by dilution plating.
C57 bl 6 or c3h hej mice
Manufactured by Jackson ImmunoResearch
C57 BL/6 and C3H/HeJ mice are inbred laboratory mouse strains commonly used in biomedical research. These mice are genetically distinct and exhibit different phenotypic characteristics. They serve as important animal models for various studies, including immunology, genetics, and disease research.
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Lab products found in correlation
2 protocols using c57 bl 6 or c3h hej mice
1
Respiratory Tract Colonization in Mice
2
Assessing Bacterial Lung Colonization in Mice
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All work with mice was conducted following institutional guidelines. Six-week-old female C57/BL6 or C3H/HeJ mice (Jackson Laboratories, Bar Harbor, Maine) were used for assessing the colonization and the progress of infection of the respiratory tract. As required, 5–150 CFU of bacteria was delivered in 5 μL of PBS to the nares of mice anesthetized with isoflurane/oxygen. For the growth curves, groups of 4 mice were infected with WT or mutant bacteria, and at the indicated days 4 mice each of WT and mutant were euthanized with carbon dioxide (CO2) and the nasal cavity, trachea, and lungs were collected in PBS and homogenized using a bead tissue disruptor. Bacterial load was enumerated by dilution plating.
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