Animals were euthanised by live decapitation with no anaesthesia after 1 or 30D of forced abstinence. Whole brains were quickly flash frozen. The NAc core was dissected by gross microdissection on dry ice. Total RNA was extracted from NAc core tissue using the Mirvana Paris Protein & RNA Isolation System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer's instructions as previously described (Sillivan et al., 2019 (link)). The RNA was suspended in RNase free water, and the concentration of the RNA was measured using the Qubit HS Assay Kit (Life Technologies Corporation of Thermo Fisher Scientific, Frederick, Maryland). One animal was excluded from the molecular analysis due to low RNA yield.
Mirvana paris protein rna isolation system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Mirvana Paris Protein & RNA Isolation System is a laboratory equipment designed for the simultaneous extraction and purification of both proteins and RNA from a single biological sample. The system utilizes a proprietary buffer and column-based approach to enable efficient and high-yield recovery of these biomolecules.
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Lab products found in correlation
2 protocols using mirvana paris protein rna isolation system
1
Extraction of NAc Core RNA
2
Rapid Brain Dissection and RNA Extraction
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Immediately after the 10th self-administration session, all animals were briefly anesthetized with 5% isoflurane and rapidly decapitated. Brains were removed and frozen in ice-cold isopentane on dry ice. Brains were kept at −80 °C until dissection. The orbitofrontal cortex (OFC) was dissected with a 1mm sample corer on a cold plate maintained at −20 °C to ensure tissue remained frozen. Frozen tissue punches were stored at −80 °C until RNA extraction. Total RNA was extracted from OFC tissue using the MIRvana Paris Protein & RNA Isolation System (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions as previously described [59 (link)]. The RNA fraction was suspended in RNase free water.
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