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Chemotx 101 5

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The ChemoTx® #101-5 is a cell migration assay system designed for chemotaxis experiments. It features a 96-well format with a polycarbonate membrane to facilitate the study of cell migration in response to chemical stimuli.

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Lab products found in correlation

Polysorbate 80, by Thermo Fisher Scientific (2 mentions) Seven compact, by Mettler Toledo (2 mentions) 68ga gacl3, by Eckert & Ziegler (2 mentions) Lgg 100, by Eckert & Ziegler (2 mentions) Hydroxyethylstarch hes200 0.5, by Biosynth (2 mentions) N formylmethionyl leucyl phenylalanine fmlp, by Santa Cruz Biotechnology (2 mentions)

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ChemoTx (29 citations)

3 protocols using chemotx 101 5

1

Measuring Macrophage Chemotaxis to MCP-1

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The chemotactic activity of peritoneal macrophages (4.0×106/mL) in response to 10 ng/mL of the chemokine monocyte chemotactic protein‐1 (M6667‐10UG, Sigma‐Aldrich Inc.) was measured in a 96‐well microchemotaxis Boyden chamber (Chemo‐Tx 101‐5; Neuro Probe Inc., Gaithersburg, MD), as described elsewhere.20, 30 Macrophages that had transmigrated through the micropore were stained with trypan blue. The number of macrophages that migrated in response to monocyte chemotactic protein‐1 was counted.
Tanaka S., Matsumoto T., Matsubara Y., Harada Y., Kyuragi R., Koga J., Egashira K., Nakashima Y., Yonemitsu Y, & Maehara Y. (2016). BubR1 Insufficiency Results in Decreased Macrophage Proliferation and Attenuated Atherogenesis in Apolipoprotein E‐Deficient Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(9), e004081.
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2

Radiolabeling Procedures for PET Imaging

Cited 1 time
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Unless otherwise indicated, reagents were obtained from Sigma-Aldrich and used without further purification. 8-hydroxyquinoline was obtained in ACS reagent grade, >99% purity. No-carrier-added, GMP-grade zirconium-89 (>150 MBq/nmol; BV Cyclotron, VU Amsterdam, NL) was purchased from PerkinElmer as [89Zr]Zr-oxalate in 1 M oxalic acid. [111In]InCl3 in 0.1 M HCl was purchased from Curium, UK. [68Ga] GaCl3 was eluted with 0.1 M HCl from a TiO2-based 68Ge/68Ga generator (lGG-100, Eckert & Ziegler). Copper-64 was prepared by 64Ni(p,n)64Cu nuclear reaction on a CTl RDS 112 11 MeV cyclotron as previously described [40 (link)]. Hydroxyethylstarch (HES200/0.5) was obtained from Carbosynth. Polysorbate 80 was obtained from Alfa Aesar. pH values were measured with a SevenCompact (Mettler-Toledo, UK) pH metre. Chemotaxis plates (polycarbonate membrane, 3.2 mm diameter, 5 μm pore size; ChemoTx® #101-5) were obtained from Neuroprobe. N- formylmethionyl-leucyl-phenylalanine (fMLP) was obtained from Santa Cruz Biotechnology. Antibodies were obtained from Miltenyi Biotec unless otherwise specified.
Man F., Khan A.A., Carrascal-Miniño A., Blower P.J, & de Rosales R.T. (2020). A kit formulation for the preparation of [89Zr]Zr(oxinate)4 for PET cell tracking: White blood cell labelling and comparison with [111In]In(oxinate)3. Nuclear medicine and biology, 90-91, 31-40.
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3

Radiolabeling Procedures for PET Imaging

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise indicated, reagents were obtained from Sigma-Aldrich and used without further purification. 8-hydroxyquinoline was obtained in ACS reagent grade, >99% purity. No-carrier-added, GMP-grade zirconium-89 (>150 MBq/nmol; BV Cyclotron, VU Amsterdam, NL) was purchased from PerkinElmer as [89Zr]Zr-oxalate in 1 M oxalic acid. [111In]InCl3 in 0.1 M HCl was purchased from Curium, UK. [68Ga] GaCl3 was eluted with 0.1 M HCl from a TiO2-based 68Ge/68Ga generator (lGG-100, Eckert & Ziegler). Copper-64 was prepared by 64Ni(p,n)64Cu nuclear reaction on a CTl RDS 112 11 MeV cyclotron as previously described [40 (link)]. Hydroxyethylstarch (HES200/0.5) was obtained from Carbosynth. Polysorbate 80 was obtained from Alfa Aesar. pH values were measured with a SevenCompact (Mettler-Toledo, UK) pH metre. Chemotaxis plates (polycarbonate membrane, 3.2 mm diameter, 5 μm pore size; ChemoTx® #101-5) were obtained from Neuroprobe. N- formylmethionyl-leucyl-phenylalanine (fMLP) was obtained from Santa Cruz Biotechnology. Antibodies were obtained from Miltenyi Biotec unless otherwise specified.
Man F., Khan A.A., Carrascal-Miniño A., Blower P.J, & de Rosales R.T. (2020). A kit formulation for the preparation of [89Zr]Zr(oxinate)4 for PET cell tracking: White blood cell labelling and comparison with [111In]In(oxinate)3. Nuclear medicine and biology, 90-91, 31-40.
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