Goat anti rabbit igg as014

Manufactured by ABclonal

Sourced in United States

Goat anti-rabbit IgG (AS014) is a secondary antibody produced in goats and specifically targets rabbit immunoglobulin G (IgG). It can be used in various immunoassay techniques for the detection and quantification of rabbit IgG in research samples.

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Lab products found in correlation

Ascorbic acid a7506, by Merck Group (1 mentions) Proteinase inhibitor cocktail 11697498001, by Roche (1 mentions) Protein g agarose fast flow beads 16 266, by Merck Group (1 mentions) Pngase f p0704s, by New England Biolabs (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions) Anti beta actin antibody 66009 1 ig, by Proteintech (1 mentions) Anti flotillin 1, by Cell Signaling Technology (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions) Anti snail, by Cell Signaling Technology (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Goat anti mouse igg as003, by ABclonal (1 mentions)

3 protocols using goat anti rabbit igg as014

Protein Expression Analysis in Cells

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Ascorbic acid (A7506) and ethyl-3,4-dihydroxybenzoate (EDHB) (E24859) were purchased from Sigma-Aldrich (Darmstadt, Germany). GM130 rabbit polyclonal antibody (A5344), P4HA1 rabbit polyclonal antibody (A3999), Rabbit polyclonal antibody anti-Laminin (LAMC1) (A16020), Rabbit anti-HA-Tag polyclonal antibody (AE036), HRP-conjugated goat anti-mouse IgG (AS003), goat anti-rabbit IgG (AS014), and HRP conjugated rabbit anti-goat IgG (AS029) were purchased from ABclonal (Cambridge, MA, USA). Goat anti-human Type I collagen (1310–01s) antibody, which was shown to react to mouse Type I collagen, was purchased from Southern Biotech (Birmingham, AL, USA),. Proteinase inhibitor cocktail (11697498001) and 4', 6-diamidino-2-phenylindole (DAPI) (10236276001) were purchased from Roche Diagnostics (Mannheim, Germany). Alexa Fluor 555 conjugated donkey anti-goat IgG (ANT082) was purchased from AntGene Biotech (Wuhan, China). Alexa Fluor 488 conjugated donkey anti-rabbit IgG (A21206)waspurchased from Invitrogen (Eugene, OR, USA). Protein G Agarose Fast Flow beads (16–266) were purchased from Merck Millipore (Darmstadt, Germany). PNGase F (P0704S) was purchased from New England BioLabs (Beverly, MA, USA).

Shi R., Hu W., Zhang Y., Gao S., Smith A.H., Ye J., Cai L., Graham L.M, & Li C. (2019). Ascorbate inducible N259 glycans on prolyl 4-hydroxylase subunit α1 promote hydroxylation and secretion of Type I collagen. Cellular and molecular life sciences : CMLS, 76(17), 3449-3464.

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Anti-Inflammatory Effects of Urokinase

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Urokinase (UK) for injection (250,000 U; Guangdong Tianpu Biochemical Pharmaceutical Co., Ltd) was obtained from Central Pharmacy of the First Affiliated Hospital of Guangxi Medical University. Sodium pentobarbital used in anesthesia was purchased from Beijing Solarbio Science & Technology Co., Ltd. Antibodies against p65 along with horseradish peroxidase‐conjugated goat anti‐rabbit antibodies and bicinchoninic acid (BCA) assay were purchased from Shanghai Biyuntian Biotechnology Co., Ltd. Anti‐TNF‐α antibodies and anti‐IL‐1β antibodies were purchased from Beijing Solarbio Technology Co., Ltd.
Anti‐IκB alpha antibody (ab32518) and anti‐phospho‐NF‐κB p65 (S536) antibody (ab86299) were purchased from Abcam. Anti‐NF‐κB p65 antibody (10745‐1‐AP) and anti‐beta‐actin antibody (66009‐1‐Ig) were purchased from Proteintech. Anti‐phospho‐p38 MAPK antibody (AP0526), anti‐p38 MAPK antibody (A4771), and goat anti‐rabbit IgG (AS014) were purchased from ABclonal Technology Co., Ltd. Anti‐phospho‐IκBα (S32) antibody (2859T) was purchased from Cell Signaling Technology. BL‐420F biological signal acquisition and analysis system was purchased from Chengdu Taimeng Software Co., Ltd. The needle of a 5 mL syringe is used for puncture.

Zhong Y., Liang B., Zhang X., Li J., Zeng D., Huang T, & Wu J. (2024). NF‐κB affected the serum levels of TNF‐α and IL‐1β via activation of the MAPK/NF‐κB signaling pathway in rat model of acute pulmonary microthromboembolism. Pulmonary Circulation, 14(2), e12357.

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Flotillin-1 and Snail Regulation

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Flotillin-1 and Snail were inserted into the pLVX vector. The Flotillin-1 shRNA sequences were cloned into the pSIH vector. The sequences of shRNAs are shown in Table S1.
Antibodies used in this study: anti-Flotillin-1 (#18634; Cell Signaling Technology, Danvers, MA, USA), anti-Snail (#3879; Cell Signaling Technology, Danvers, MA, USA), anti-E-cadherin (#3195; Cell Signaling Technology, Danvers, MA, USA), anti-N-cadherin (#13116; Cell Signaling Technology, Danvers, MA, USA), anti-Vimentin (#5741; Cell Signaling Technology, Danvers, MA, USA), anti-β-actin (A5441; Sigma-Aldrich, USA), anti-Flag (#14793; Cell Signaling Technology, Danvers, MA, USA), anti-HA (sc-53516; Santa Cruz Biotechnology, Dallas, TX, USA), Goat anti-Rabbit IgG (AS014; ABclonal, Hubei, China), Goat anti-mouse IgG (AS003; ABclonal, Hubei, China).

Huang Y., Guo Y., Xu Y., Liu F, & Dai S. (2022). Flotillin-1 promotes EMT of gastric cancer via stabilizing Snail. PeerJ, 10, e13901.

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