Rat anti mouse il 10 receptor igg1 monoclonal antibody αil 10r

Rat anti-mouse IL-10 receptor (IgG₁) monoclonal antibody (αIL-10R) and rat anti-mouse PD-1 monoclonal antibody were purchased from BioXcell (West Lebanon, NH). Hexadecyltrimethylammonium bromide, hydrogen peroxide, human neutrophil myeloperoxidase (MPO), lipopolysaccharide (LPS), RNAlater^®, TRI Reagent^® were purchased from Sigma (St. Louis, MO). Guaiacol (2-methoxyphenol) was obtained from Alfa Aesar (Ward Hill, MA). Mouse lipocalin 2 (Lcn2) and keratinocyte-derived chemokine CXCL1 (KC) duoset ELISA kits were procured from R&D Systems (Minneapolis, MN). SYBR^® Green mix was procured from Quanta Biosciences (Gaithersburg, MD).

Reagent grade Dextran Sulfate Sodium salt (DSS, reagent grade, M.W 36–50kDa, Ref = 160110) was purchased from MP Biomedicals (Solon, OH). Duoset ELISA kits for mouse and human Lcn2 and keratinocyte-derived chemokine (KC) were obtained from R&D Systems (Minneapolis, MN). α-Hu-Lcn2 SYBR® Green mix and qScript cDNA synthesis kit was procured from Quanta Biosciences (Gaithersburg, MD). Guaiacol (2-methoxyphenol) was obtained from Alfa Aesar (Ward Hill, MA). Myeloperoxidase (MPO) was procured from Sigma (St. Louis, MO). Ly6G antibody was purchased from Abcam (Cambridge, MA). Rat anti-mouse IL-10 receptor (IgG₁) monoclonal antibody (αIL-10R) and rat anti-mouse PD-1 monoclonal antibody (IgG1 isotype control) were procured from BioXcell (West Lebanon, NH). Anti-mouse CD45-Alexafluor 594 and CD326 monoclonal antibodies were procured from BioLegend (San Diego, CA) RegIIIγ and Ang4 antibodies are kind gift from Dr. Lora Hooper, UT Southwestern Medical Center, TX. All other fine chemicals used in present study were reagent grade and procured from Sigma.