Native gel electrophoresis experiments were performed on 20 % polyacrylamide gels containing TBE (8.9 mm Tris, 8.9 mm borate, 0.2 mm EDTA, from BIORAD) and 30 mm KCl, at room temperature, 120 V for 2 h. The ODN samples, annealed at 1.6 mm single strand concentration in 1.0 m K+ buffer, were diluted at 0.6 mm loading concentration just before the PAGE runs. Glycerol was added (10 % final) to facilitate sample loading in the wells. The bands were finally visualized by ethidium bromide staining in a Bio‐Rad Laboratories Gel DocTM XR+ image system.
Gel doctm xr image system
Manufactured by Bio-Rad
Sourced in Italy
The Gel Doc XR+ image system is a high-performance gel documentation system designed for capturing and analyzing DNA, RNA, and protein gels. It features a sensitive CCD camera, motorized zoom lens, and a versatile sample tray that can accommodate a variety of gel sizes and types. The system provides accurate and reproducible image acquisition, enabling researchers to visualize and analyze their experimental results.
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Lab products found in correlation
Ethylene diamine tetraacetic acid (edta), by Bio-Rad (1 mentions)
Ptc 348 wi temperature, by Jasco (1 mentions)
2075 plus, by Jasco (1 mentions)
Unity inova 700 mhz spectrometer, by Agilent Technologies (1 mentions)
V 530 spectrophotometer, by Jasco (1 mentions)
Unity inova 500 mhz spectrometer, by Agilent Technologies (1 mentions)
2 protocols using gel doctm xr image system
1
Native Gel Electrophoresis of ODN Samples
2
Chemically Synthesized DNA Oligonucleotides
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Chemicals and solvents were purchased from Sigma-Aldrich (Milan, Italy). Reagents and phosphoramidites for DNA syntheses were purchased from Glen Research (Sterling, VA, USA). The DEL linker 2 , the TEL linker 3 and all the ODNs were assembled on a PerSeptive Biosystems (Framingham, MA, USA) Expedite DNA synthesizer using standard phosphoramidite chemistry. HPLC analyses and purifications were performed with a Jasco (Jasco Europe Srl, Cremella, LC, Italy) PU2089 pump system equipped with an UV detector model 2075 Plus. CPG-resin 1 and the linkers 4 and 5 were purchased from Glen Research. UV spectra were registered using a Jasco V-530 spectrophotometer. CD spectra and thermal denaturation experiments were obtained with a Jasco 1500 CD spectrophotometer equipped with a Jasco PTC-348-WI temperature controller unit. NMR spectra were recorded either on a Varian (Palo Alto, CA, USA) Unity Inova 500 MHz spectrometer equipped with a broadband inverse probe with z-field gradient, or on a Varian Unity INOVA 700 MHz spectrometer equipped with a triple resonance cryoprobe. All NMR spectra were processed using the Varian VNMR and iNMR (http://www.inmr.net ) software packages. PAGE bands were visualized on a Bio-Rad Laboratories (Segrate, MI, Italy) Gel DocTM XR+ image system.
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