Adviaxpt 2400

Manufactured by Siemens

Sourced in Germany

The AdviaXPT 2400 is a laboratory analyzer that performs automated clinical chemistry and immunoassay testing. It is designed for high-volume testing environments, offering efficient sample processing and reliable results.

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Lab products found in correlation

Tr0100, by Merck Group (1 mentions) Mithras microplate reader, by Berthold Technologies (1 mentions) Ch200, by Randox (1 mentions) Sircol soluble collagen assay kit, by Biocolor (1 mentions)

2 protocols using adviaxpt 2400

Biomarker Evaluation in Chronic Kidney Disease

Cited 1 time

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At baseline and after 12 and 24 months, blood was drawn and urine was taken under fasting conditions in the morning and samples were immediately processed in the accredited Central Laboratory of Heidelberg University Hospital. Plasma and/or urine levels of creatinine, cystatin C, total cholesterol, lipoprotein a, HDL-cholesterol, triacylglycerols and albumin in all participants were analysed with clinical chemistry automation (AdviaXPT 2400 chemistry analyser, Siemens Healthineers, Germany) according to the appropriate standard operating protocol. Albumin/creatinine ratio (urinary ACR) was calculated in mg/g and values >30 mg/g were defined as pathological. The Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula was used for the estimation of GFR [20 (link)].

Morgenstern J., Groener J.B., Jende J.M., Kurz F.T., Strom A., Göpfert J., Kender Z., Le Marois M., Brune M., Kuner R., Herzig S., Roden M., Ziegler D., Bendszus M., Szendroedi J., Nawroth P., Kopf S, & Fleming T. (2021). Neuron-specific biomarkers predict hypo- and hyperalgesia in individuals with diabetic peripheral neuropathy. Diabetologia, 64(12), 2843-2855.

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Quantification of Liver Lipids and Collagen

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Triglycerides (TR0100, Sigma Aldrich) and total cholesterol (CH200, Randox, Crumlin, UK) were measured in serum and liver lipid lysates using the Mithras Microplate Reader (Berthold Technologies GmbH & Co, Bad Wildbad, Germany) at 550 nm. Liver lipids were extracted as described previously [15 ]. Serum levels of HDL-cholesterol were analyzed with an AdviaXPT® 2400 clinical chemistry automation (Siemens Healthineers) according to the standard operating protocol. Sircol soluble collagen assay kit (Biocolor life science assays) was used to quantify collagen in mouse liver tissue according to the manufacturer's instructions. Briefly, approximately 25–50 mg of liver material was digested with pepsin overnight at 4 °C. Neutralized samples were concentrated and mixed with Sirius red dye reagent for 30 min before washing and centrifugation. Alkali reagent was added to release collagen bound dye into solution. Samples were measured on the Mithras Microplate Reader at 555 nm.

Wolff G., Sakurai M., Mhamane A., Troullinaki M., Maida A., Deligiannis I.K., Yin K., Weber P., Morgenstern J., Wieder A., Kwon Y., Sekar R., Zeigerer A., Roden M., Blüher M., Volk N., Poth T., Hackert T., Wiedmann L., De Angelis Rigotti F., Rodriguez-Vita J., Fischer A., Mukthavaram R., Limphong P., Tachikawa K., Karmali P., Payne J., Chivukula P., Ekim-Üstünel B., Martinez-Jimenez C.P., Szendrödi J., Nawroth P, & Herzig S. (2022). Hepatocyte-specific activity of TSC22D4 triggers progressive NAFLD by impairing mitochondrial function. Molecular Metabolism, 60, 101487.

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