The p53 firefly luciferase reporter plasmid (pp53-TA-luc; Beyotime Biotechnology) and the internal control Renilla luciferase reporter plasmid (Promega) were cotransfected into HepG2 using Lipofectamine 2000 according to supplier’s directions. After transfection, the transfected cells were treated butein for 24 h and the luciferase activities in cell lysates were determined with the Dual Luciferase Reporter Assay kit (Promega).
Pp53 ta luc
Manufactured by Beyotime
Sourced in United States
The Pp53-TA-luc is a plasmid that contains a p53-responsive luciferase reporter gene. It is designed to monitor p53 transcriptional activity in cells.
Automatically generated - may contain errors
2 protocols using pp53 ta luc
1
Evaluation of Butein's Effect on p53 Transcriptional Activity
2
Lentiviral Constructs and RNA Interference
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lentivirus-circSCAP and lentivirus-vector were purchased from GenePharma (Suzhou, China). Lentiviruses were ultracentrifuged, concentrated, and validated. A specific small interfering RNA for hsa_circ_0065214 was designed to target the covalent closed junction. All si RNAs, si NC (negative control), microRNA (miRNA) mimics and inhibitors were also purchased from GenePharma (Suzhou, China) (Table S1 ). PcDNA3.1( +)-SF3A3 (Umine Biotechnology Co., LTD., Guangzhou, China), pmirGLO- hsa_circ_0065214 (GeneCopoeia, USA), and pp53-TA-luc (Beyotime Biotechnology, Shanghai, China) and pySEAP (Kelei biological Technology Co., LTD., China) were transformed into DH5α competent cells, coated plates, and selected monoclonal colony to multiply. All of them were transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).
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