We used size exclusion chromatography (SEC) to assess the oligomerization state of the purified MBP-FL Vpu, MBP-VpuΔh2h3, and MBP-VpuΔh3 proteins, as described previously. A buffer composed of 50 mM sodium phosphate (NaPi) pH 7.4, 150 mM NaCl, 200 μM TCEP and 10% glycerol was used to equilibrate the column prior to protein sample injection. Then 500 μL of protein sample was injected into the column and eluted by running a SEC program with 0.5 ml/min flow rate. For these experiments, we used a Superdex™ 200 increase 10/300 GL size-exclusion column (GE Healthcare) plugged into an AKTÄ explorer 100 (Amersham Biosciences) protein purifier system.
Akt explorer 100
Manufactured by Cytiva
The ÄKTA explorer 100 is a compact, flexible, and versatile chromatography system designed for fast and easy purification of biomolecules. It features user-friendly software, pre-programmed methods, and a compact footprint, making it suitable for a wide range of applications in research and development laboratories.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using akt explorer 100
1
Oligomerization Analysis of Vpu Proteins
2
Oligomerization Assessment of MBP-Vpu Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used size exclusion chromatography (SEC) to assess the oligomerization state of the purified MBP-FL Vpu, MBP-VpuΔh2h3, and MBP-VpuΔh3 proteins, as described previously. A buffer composed of 50 mM sodium phosphate (NaPi) pH 7.4, 150 mM NaCl, 200 µM TCEP and 10% glycerol was used to equilibrate the column prior to protein sample injection. Then 500 µL of protein sample was injected into the column and eluted by running a SEC program with 0.5 ml/min flow rate. For these experiments, we used a Superdex™ 200 increase 10/300 GL size-exclusion column (GE Healthcare) plugged into an AKTÄ explorer 100 (Amersham Biosciences) protein purifier system.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!