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Texas red labeled anti rabbit igg

Manufactured by Vector Laboratories
Sourced in United Kingdom

Texas Red labeled anti-rabbit IgG is a secondary antibody reagent used for immunodetection. It is conjugated with the fluorescent dye Texas Red, which allows for visualization and detection of rabbit primary antibodies in various immunoassay applications.

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2 protocols using texas red labeled anti rabbit igg

1

Immunofluorescence Staining of CX3CR1 in HAE Cultures

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Cross-sections of formalin fixed and paraffin embedded HAE cultures were subjected to antigen retrieval (BioGenex, Fremont, CA) prior to incubation with polyclonal rabbit CX3CR1-specific IgG antibody ab8020 (Abcam, Cambridge, UK) or isotype control ab27478 (Abcam) followed by Texas Red labeled anti-rabbit IgG (Vector Laboratories, Burlingame, CA) and ProLong Gold anti-fade reagent with DAPI (Life Technologies, Carsbad, CA). Images were taken on an Olympus BX61 microscope (Olympus Corporation, Tokyo, Japan).
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2

Labeling of Anti-MCMV Protein with FITC

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Anti-MCMV early antigen (EA) [38 (link)] was labeled with fluorescein isothiocyanate (FITC; Sigma-Aldrich, St. Louis, MO) as previously described [39 (link)]. Anti-RPE65 was kindly provided by Dr. Michael Redmond (National Eye Institute, National Institutes of Health, Bethesda, MD). Anti-caspase 1, antiphosphorylated NFκB (p-p65), anti-RIP1, anti-RIP3, goat anti-rabbit immunoglobulin G horseradish peroxidase (IgG-HRP), and goat anti-mouse IgG-HRP were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Anti-β-actin was purchased from Sigma-Aldrich (St. Louis, MO). Texas Red–labeled anti-rabbit IgG was obtained from Vector Laboratories (Burlingame, CA).
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