Human normal bronchial epithelial cell line BEAS-2B and LC cell lines Calu3, A549 and HCC827 were bought from American Type Culture Collection (ATCC; ATCC CRL-9609, ATCC HTB-55, ATCC CCL-185, ATCC CRL-2868, Rockefeller, MD, USA). A separate LC cell line, PC-9, was obtained from Shanghai Yaji Biological Technology Co., Ltd. (YS319C, China, http://www.yajimall.com/ ). BEAS-2B was incubated in bronchial epithelial growth medium (BEGM; CC-3170, Lonza/Clonetics Corporation, USA) in which we removed the Gentamycin-Amphotericin B mix (GA-1000). Calu3 cells were cultured in Eaglés Minimum Essential Medium (EMEM; ATCC 30-2003, ATCC, USA) while A549 cells were incubated in Kaighn's Modification of Ham's F-12 (F-12K) Medium (ATCC 30-2004). PC-9 and HCC827 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (ATCC 30-2001, ATCC). All media except BEGM were supplemented with 10% fetal bovine serum (FBS; ATCC 30-2020, USA) and 1% Penicillin-Streptomycin Solution (ATCC 30-2300, ATCC). All cells were incubated in a humidified atmosphere at 37°C with 5% CO2.
Atcc crl 9609
Manufactured by American Type Culture Collection
Sourced in United States
ATCC CRL-9609 is a cell line derived from human lung carcinoma tissue. This cell line is suitable for research applications that require a human lung carcinoma cell model.
Automatically generated - may contain errors
Lab products found in correlation
Atcc ccl 185, by American Type Culture Collection (1 mentions)
Atcc htb 55, by American Type Culture Collection (1 mentions)
Calu 3, by American Type Culture Collection (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
Cc 3170, by Lonza (1 mentions)
Beas 2b, by Lonza (1 mentions)
Cc 3171, by Lonza (1 mentions)
Cc 4175, by Lonza (1 mentions)
Penicillin, by Harvard Bioscience (1 mentions)
Streptomycin, by Harvard Bioscience (1 mentions)
Sodium pyruvate, by PAN Biotech (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
N hexane, by Avantor (1 mentions)
L glutamine, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Fetal calf serum (fcs), by PAN Biotech (1 mentions)
Rpmi 1640 medium, by PAN Biotech (1 mentions)
3 protocols using atcc crl 9609
1
Culturing Diverse Lung Cell Lines
2
Culturing BEAS-2B Cells for Functional Assays
3
Artemisia annua L. Leaf and Flower Protocol
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Artemisia annua L. plant was used as raw material and its leaves and flowering branches of aerial parts were collected by Yasam Pharma from (40.178915, 29.141146 ) coordinates of Uludağ in Bursa during the semi-flowering period in the end of summer 2020.
Ethanol (Merck, USA), MEthanol (Merck, USA), n-Hexane (VWR Chemicals, USA), Sodiumhidroxide (IsoLab, Germany) and Acetic acid (IsoLab, Germany) were used in maceration and derivatization reactions and selected as analytical grade purity. Artemisinin chromatography grade standard (CAS: 63968-64-9) was purchased from Sigma-Aldrich, USA.
Beas-2B (Epithelial cells isolated from normal human bronchial epithelium, ATCC® CRL-9609™) was used for in vitro cell culture study in scope of cytotoxic and genotoxic analysis. Culture medium was prepared with RPMI 1640 medium (PAN Biotech, Germany), 10% Fetal Calf serum (PAN Biotech, Germany), 1 mM Sodium Pyruvate (PAN Biotech, Germany), 1 mM L-Glutamine (Sigma Aldrich, USA), 100 U/ml Penicillin -100 μg/ ml Streptomycin (Biochrom AG, Germany).
Ethanol (Merck, USA), MEthanol (Merck, USA), n-Hexane (VWR Chemicals, USA), Sodiumhidroxide (IsoLab, Germany) and Acetic acid (IsoLab, Germany) were used in maceration and derivatization reactions and selected as analytical grade purity. Artemisinin chromatography grade standard (CAS: 63968-64-9) was purchased from Sigma-Aldrich, USA.
Beas-2B (Epithelial cells isolated from normal human bronchial epithelium, ATCC® CRL-9609™) was used for in vitro cell culture study in scope of cytotoxic and genotoxic analysis. Culture medium was prepared with RPMI 1640 medium (PAN Biotech, Germany), 10% Fetal Calf serum (PAN Biotech, Germany), 1 mM Sodium Pyruvate (PAN Biotech, Germany), 1 mM L-Glutamine (Sigma Aldrich, USA), 100 U/ml Penicillin -100 μg/ ml Streptomycin (Biochrom AG, Germany).
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