Penicillin streptomycin solution

Manufactured by Yeasen

Sourced in United States, China

Penicillin-streptomycin solution is a sterile liquid mixture of the antibiotics penicillin and streptomycin. It is commonly used in cell culture applications to prevent bacterial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Lentiviral vector, by Genomed (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Nci h1299, by National Collection of Authenticated Cell Cultures (1 mentions) Nci h460, by National Collection of Authenticated Cell Cultures (1 mentions) Dodecanthiol, by Merck Group (1 mentions) Cell counting kit cck 8, by Yeasen (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)

4 protocols using penicillin streptomycin solution

MRC-5 Cell Culture Protocol

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MRC-5 cells were cultured in MEM (Gibco) supplemented with 1% nonessential amino acid solution (BI), 10% fetal bovine serum (Gibco), 1% penicillin/streptomycin solution (Yeasen), and 1% sodium pyruvate solution (BI). Cells were maintained in an incubator at 37 °C under 5% CO₂.

Liu W., Lin H., Mao Z., Zhang L., Bao K., Jiang B., Xia C., Li W., Hu Z, & Li J. (2020). Verapamil extends lifespan in Caenorhabditis elegans by inhibiting calcineurin activity and promoting autophagy. Aging (Albany NY), 12(6), 5300-5317.

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Overexpression and Knockdown of circUSP7 in NSCLC

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The NSCLC cell lines NCI-H460, NCI-H1299, A549, PC9, and 95D and the normal human bronchial epithelial cell line HBE were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640 or DMEM (HyClone, Logan City, USA) supplemented with 10% foetal bovine serum (FBS) (Gibco, USA) and 1% penicillin-streptomycin solution (Yeasen, China) was used to culture the NSCLC cells at 37 °C in a humidified atmosphere containing 5% CO₂. All the lentiviral vectors used in this study were obtained from Genomeditech (Shanghai, China). The circUSP7-overexpression and circUSP7-short hairpin RNA (shRNA) lentiviral vectors were transfected into NSCLC cells according to the manufacturer’s instructions.

Chen S.W., Zhu S.Q., Pei X., Qiu B.Q., Xiong D., Long X., Lin K., Lu F., Xu J.J, & Wu Y.B. (2021). Cancer cell-derived exosomal circUSP7 induces CD8+ T cell dysfunction and anti-PD1 resistance by regulating the miR-934/SHP2 axis in NSCLC. Molecular Cancer, 20, 144.

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Synthesis and Characterization of Magnetic Nanoparticles

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Methacrylic acid (MAA, 99%), 2,2′-azobisisobutyronitrile (AIBN, 98%), anhydrous ethanol, Ferric chloride (FeCl₃·6H₂O, 99%), ferrous sulfate (FeSO₄·7H₂O, 99%), hydrochloric acid (HCl, 38%), ammonium hydroxide (NH₄OH, 28%), anhydrous acetone, and anhydrous diethyl ether were purchased from National Medicines Corporation Ltd. of P. R. China (Beijing, China). Dodecanthiol (DDT, 99%) was purchased from Sigma-Aldrich (Shanghai, China). RPMI Medium 1640, Dulbecco’s modified eagle’s medium (DMEM), and phosphate buffer saline (PBS) were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Gibco (Basel, Switzerland). Trypsin-EDTA (0.25%) phenol red, Penicillin-Streptomycin Solution, Cell Counting Kit (CCK-8) were purchased from Yeasen (Shang Hai, China).

Xiang C., Zhong X., Yang W., Majeed M.I., Wang J., Yu J., Hu J., Xu Z., Tan B., Zhang B, & Yan W. (2019). Fe3O4 Nanoparticles Functionalized with Polymer Ligand for T1-Weighted MRI In Vitro and In Vivo. Polymers, 11(5), 882.

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Investigating Macamide B's Role in Lung Cancer

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Human lung cancer cell lines,H460, H1299 and A549, were purchased from Beyotime Institute of Biotechnology and were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin solution (Shanghai Yeasen Biotechnology Co., Ltd.) under an atmosphere of 5% CO₂ at 37°C. H460 and A549 cell lines contain the p53 gene, whereas the H1299 cell line lacks the p53 gene. The two types of cell lines therefore may better reflect the role of macamide B in lung cancer. Macamide B (Chemical Abstracts Service no., 74058-71-2) was obtained from Shanghai Yuanye Biotechnology Co., Ltd. and dissolved in DMSO (Gibco; Thermo Fisher Scientific, Inc.). Small interfering RNA (siRNA) oligonucleotides targeting the ataxia-telangiectasia mutated (ATM) gene (siATM) were designed and chemically synthesized by General Biosystems (Anhui) Co., Ltd. The siATM sequence was as follows: 5'-AGGTGCTTATGAATCAACAAAAT-3'. The siRNA negative control (siNC) sequence was as follows: 5'-AACACCGAACGAGACACGATT-3'.

Tao H., Shi H., Wang M, & Xu Y. (2023). Macamide B suppresses lung cancer progression potentially via the ATM signaling pathway. Oncology Letters, 25(3), 115.

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