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E 450

Manufactured by Olympus
Sourced in Japan

The E-450 is a high-performance laboratory equipment designed for various applications. It features advanced technology and precise controls to ensure reliable and consistent results.

Automatically generated - may contain errors

3 protocols using e 450

1

Rice Image Collection and Transmission

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The experimental field images analyzed in this work were captured in Jiangxi and Guangxi Provinces, China. All the images were taken under natural illumination from 2010 to 2013. The imaging device includes an image-capturing and a communication system [43 (link)], as shown in Figure 2. The image-capturing system is used for data collection. In detail, rice image sequences (4272 × 2848) are captured in Jiangxi with an OLYMPUS E-450 camera during the daytime from 9:00 to 16:00 every hour within 2010 to 2013. Similarly, rice images with resolution 3648 × 2736 are also captured in Guangxi with a Canon EOS 1100D camera during the daytime from 7:00 to 19:00 every hour within 2012 to 2013. Moreover, the communication system including the antenna and encoder is used for data transmission through 3G wireless networks.
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2

Comprehensive Characterization of Lichen Specimens from South Korea

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The lichen specimens were collected from Seonam temple, Jeollanam Province, South Korea from 2016 to 2017 and deposited in the Korean Lichen Research Institute (KoLRI) (Fig. 1). External morphological characteristics of lichen specimens were examined using a stereomicroscope (SZX-7; Olympus, Tokyo, Japan). Macro photographs were taken with an Olympus E450 camera using the Quick Photo Camera 2.3 software. The anatomy of the thalli and ascigerous areas was examined using a microscope (Eclipse Ni-U; Nikon, Tokyo, Japan) equipped with a Nikon DS-Fi1c camera, which was operated using the NIS-Elements BR software. Calcium oxalate crystals were identified using 25% H2SO4. Spot tests of lichen specimens were conducted using 10% KOH (K), saturated Ca(OCl)2 (C), 10% KOH followed by saturated Ca(OCl)2 (KC), and 5% alcoholic p-phenylenediamine (P). Secondary metabolites of lichen specimens were identified using high-performance thin-layer chromatography (HPTLC) and thin-layer chromatography (TLC). HPTLC was performed using solvent systems A, B, and C [8 ]. TLC was performed using solvent systems A and C per the standard method [9 ]. Localities from which lichen specimens were collected were mapped using the open source GIS software Quantum GIS 1.7.4 (QGIS).
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3

Microscopic Analysis of Breast Cancer Cell Morphometry

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Images of microscopic slides, stained routinely with H and E, were captured using BH-2 microscope (OLYMPUS, Japan) equipped with a digital camera (OLYMPUS, E-450). Five images per slide were taken, under the magnification 400; the images were further analyzed using ImageJ software (imagej.nih.gov/ij/). The measured parameters included the distance between groups of cancer cells (μm; 10 shortest distances per captured image), and the area, perimeter (B), Feret's diameter and circularity (Cir) of the cells/nuclei. For the five images taken per slide, with minimum five slides per case, 15 cells/nuclei were measured. The analyzed cells had a clear cell membrane and did not overlap with the surrounding cells. The fields that were taken into consideration were on the periphery of tumor tissue where the boundary with normal breast tissue was visible.
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