For quantitative kinetics analysis, droplets of TDP-43PrLD – RNA were formed by incubating 10 μM of TDP-43PrLD in 20 mM MES buffer at pH 6.0 with 50 μg/mL RNA for 10 minutes at room temperature. To this, αS monomers, 10% of which were labeled with HiLyte 647 dye, were added. At specific time points, the samples were sedimented by centrifugation for 20 minutes at 19,000xg, and the supernatant was measured for the concentration of αS based on absorbance at 650 nm using Cary 300 Varian UV spectrophotometer. Data was processed using Origin 8.5 for at least three independent measurements.
Cary 300 varian uv spectrophotometer
Manufactured by Agilent Technologies
The Cary 300 Varian UV spectrophotometer is a laboratory instrument designed for ultraviolet-visible (UV-Vis) spectroscopy. It measures the absorption or transmission of light in the UV and visible regions of the electromagnetic spectrum, providing information about the chemical composition and properties of samples.
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2 protocols using cary 300 varian uv spectrophotometer
1
Quantitative Kinetics Analysis of TDP-43PrLD-RNA Droplets
2
Quantitative Kinetics of TDP-43PrLD–RNA Droplets
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For quantitative kinetics analysis, droplets of TDP-43PrLD – RNA were formed by incubating 10 µM of TDP-43PrLD in 20 mM MES buffer at pH 6.0 with 50 µg/mL RNA for 10 minutes at room temperature. To this, αS monomers, 10% of which were labeled with HiLyte 647 dye, were added. At specific time points, the samples were sedimented by centrifugation for 20 minutes at 19,000xg, and the supernatant was measured for the concentration of αS based on absorbance at 650 nm using Cary 300 Varian UV spectrophotometer. Data was processed using Origin 8.5 for at least three independent measurements.
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