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Anti pe or apc beads

Manufactured by Miltenyi Biotec
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Anti-PE or -APC beads are magnetic particles coated with antibodies specific to the fluorescent dyes phycoerythrin (PE) or allophycocyanin (APC). These beads can be used to isolate or enrich cells or molecules labeled with PE or APC from complex samples.

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Lab products found in correlation

Ls column, by Miltenyi Biotec (3 mentions)

Spelling variants of this product

Anti-PE beads (43 citations) Anti-APC beads (12 citations) Anti-PE or anti-APC magnetic beads (3 citations) Anti-PE or anti-APC beads (2 citations) Anti-PE or APC magnetic beads (2 citations)

3 protocols using anti pe or apc beads

1

Identification of HCV-specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC samples were thawed and analyzed by flow cytometry to identify virus-specific CD8+ T cell responses using appropriate HLA class I multimers, pre- and post-DAA treatment (Extended Data Fig. 1a,b). Magnetic bead enrichment of multimer-positive cells using anti-PE or -APC beads and LS columns (Miltenyi) was performed on up to 200 million PBMCs, according to the manufacturer’s instructions, to capture HCV-specific CD8+ T cell populations of even extremely low-frequency (Extended Data Fig. 1c).
Tonnerre P., Wolski D., Subudhi S., Al-Jabban J., Hoogeveen R.C., Damasio M., Drescher H.K., Bartsch L.M., Tully D.C., Sen D.R., Bean D.J., Brown J., Torres-Cornejo A., Robidoux M., Kvistad D., Alatrakchi N., Cui A., Lieb D., Cheney J.A., Gustafson J., Lewis-Ximenez L.L., Massenet-Regad L., Eisenhaure T., Aneja J., Haining W.N., Chung R.T., Hacohen N., Allen T.M., Kim A.Y, & Lauer G.M. (2021). Differentiation of exhausted CD8 T cells after termination of chronic antigen stimulation stops short of achieving functional T cell memory. Nature immunology, 22(8), 1030-1041.
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2

Identification of HCV-specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC samples were thawed and analyzed by flow cytometry to identify virus-specific CD8+ T cell responses using appropriate HLA class I multimers, pre- and post-DAA treatment (Extended Data Fig. 1a,b). Magnetic bead enrichment of multimer-positive cells using anti-PE or -APC beads and LS columns (Miltenyi) was performed on up to 200 million PBMCs, according to the manufacturer’s instructions, to capture HCV-specific CD8+ T cell populations of even extremely low-frequency (Extended Data Fig. 1c).
Tonnerre P., Wolski D., Subudhi S., Al-Jabban J., Hoogeveen R.C., Damasio M., Drescher H.K., Bartsch L.M., Tully D.C., Sen D.R., Bean D.J., Brown J., Torres-Cornejo A., Robidoux M., Kvistad D., Alatrakchi N., Cui A., Lieb D., Cheney J.A., Gustafson J., Lewis-Ximenez L.L., Massenet-Regad L., Eisenhaure T., Aneja J., Haining W.N., Chung R.T., Hacohen N., Allen T.M., Kim A.Y, & Lauer G.M. (2021). Differentiation of exhausted CD8 T cells after termination of chronic antigen stimulation stops short of achieving functional T cell memory. Nature immunology, 22(8), 1030-1041.
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3

Enrichment of HCV-Specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC samples were thawed and analyzed by flow cytometry to identify virus-specific CD8+ T cell responses using appropriate HLA class I multimers, pre-and post-DAA treatment (Extended Data Fig. 1a,b). Magnetic bead enrichment of multimer-positive cells using anti-PE or -APC beads and LS columns (Miltenyi) was performed on up to 200 million PBMCs, according to the manufacturer's instructions, to capture HCV-specific CD8+ T cell populations of even extremely low-frequency (Extended Data Fig. 1c).
Tonnerre P., Wolski D., Subudhi S., Aljabban J., Hoogeveen R.C., Damasio M., Drescher H.K., Bartsch L.M., Tully D.C., Sen D.R., Bean D.J., Brown J., Torres-Cornejo A., Robidoux M., Kvistad D., Alatrakchi N., Cui A., Lieb D., Cheney J.A., Gustafson J.L., Lewis‐Ximenez L.L., Massenet-Regad L., Eisenhaure T., Aneja J., Haining W.N., Chung R.T., Hacohen N., Allen T.M., Kim A.Y., & Lauer G.M. (2021). Differentiation of exhausted CD8 T cells after termination of chronic antigen stimulation stops short of achieving functional T cell memory.
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