U251 human glioblastoma cell line genetically engineered to express firefly luciferin (fLuc) was cultured in DMEM- high glucose media (Corning, NY) supplemented with 10% FBS (Invitrogen, Carlsbad, CA) and 1% Pen-Strep (Gibco, Grand Island, New York). PDX cell line was cultured in serum free neurobasal media (ThermoFisher Scientific, Somerset, NJ) supplemented with 1% anti-anti (ThermoFisher Scientific, Somerset, NJ), 50 μg human epithelial growth factor (hEFG) and 50 μg human fibroblast growth factor beta (hFGF-β).
Dmem high glucose media
Manufactured by Corning
DMEM high glucose media is a cell culture medium that provides high levels of glucose to support the growth and proliferation of various cell types. It is a commonly used basal medium in cell culture research and applications.
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Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Medium 199, by Thermo Fisher Scientific (2 mentions)
A375 melanoma, by American Type Culture Collection (2 mentions)
Glutamax supplement, by Thermo Fisher Scientific (2 mentions)
Heparin sodium salt, by Merck Group (2 mentions)
Hepes, by Thermo Fisher Scientific (2 mentions)
Endothelial cell growth supplement (ecgs), by Merck Group (2 mentions)
Crl 2279, by Corning (2 mentions)
Crl 1619, by American Type Culture Collection (2 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions)
Crl 1573, by Corning (2 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hyclone fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Rpmi 1640 media, by Corning (1 mentions)
5 protocols using dmem high glucose media
1
Culturing U251 and PDX Cell Lines
2
Doxorubicin-induced Apoptosis in HFFs
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Human neonatal foreskin fibroblasts (HFFs) (ATCC) were maintained in DMEM high glucose media (Corning) supplemented with 10% HyClone fetal bovine serum (Thermo Scientific) at 37 °C with 5% CO2. Cells were seeded at the density of 1 × 103/cm2 in 10 cm culture dish before treatment. 48 h after seeding, cells were incubated in complete medium supplemented with different concentrations of doxorubicin (Sigma-Aldrich) for 12 h. Cells were then cultured in fresh complete medium with regular medium change and subjected to staining and flow cytometry analysis after 4 and 8 days of treatment.
3
Culturing Diverse Cell Lines for Research
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A375 melanoma, Mile Sven 1 (MS1, mouse pancreatic endothelial) and HEK293 cell lines were purchased from ATCC (CRL-1619, CRL-2279 and CRL-1573 respectively) and maintained in DMEM high-glucose media (Corning, 10-013-CV) with heat inactivated FBS 10 % and Penicillin-Streptomycin (Life technologies, 15070063) 1 %. Human umbilical vein endothelial cells (HUVECs) were purchased from Yale Vascular Biology and Therapeutics program and maintained in Medium 199 (Life technologies, 11150-059) with heat inactivated FBS (Life technologies, 16140-071) 20 %, Endothelial cell growth supplement 0.03 mg/ml (Sigma-aldrich, E2759), Heparin sodium salts (Sigma-aldrich, H3149-100KU) 0.05 mg/ml, HEPES (Life technologies, 15630106) 10 mM, GlutaMAX supplement (Life technologies, 35050061) 1X and Antibiotic-Antimycotic (Life technologies, 15240112) 1 %. 0.1 % gelatin was coated for 10 minutes at 37 ℃ incubator before seeding HUVECs on the culture dish. All cell lines were cultured in humidified 37 °C and 5 % CO2 incubator.
4
Cell Culture Protocols for Hematological and Epithelial Cell Lines
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HL60 cell was maintained in IMDM media (HyClone, Cat. No. SH30228.01) with 20% fetal bovine serum (Hyclone, Cat. No. SH30910.03). THP-1 cell was maintained in RPMI-1640 media (Corning, Cat. No. 10-040-CM) with 10% fetal bovine serum and 0.05 mM β-mercaptoethanol (Fisher scientific, Cat. No. BP176-100). K562 cell was maintained in RPMI-1640 media with 10% fetal bovine serum. 293T-ecotropic and 293T-Amphotropic were maintained in DMEM high glucose media (Corning, Cat. No. 10-013-CV) with 10% fetal bovine serum. All cells were incubated at 37 degree Celsius and 5% CO2.
5
Culturing Diverse Cell Lines for Research
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A375 melanoma, Mile Sven 1 (MS1, mouse pancreatic endothelial) and HEK293 cell lines were purchased from ATCC (CRL-1619, CRL-2279 and CRL-1573 respectively) and maintained in DMEM high-glucose media (Corning, 10-013-CV) with heat inactivated FBS 10 % and Penicillin-Streptomycin (Life technologies, 15070063) 1 %. Human umbilical vein endothelial cells (HUVECs) were purchased from Yale Vascular Biology and Therapeutics program and maintained in Medium 199 (Life technologies, 11150-059) with heat inactivated FBS (Life technologies, 16140-071) 20 %, Endothelial cell growth supplement 0.03 mg/ml (Sigma-aldrich, E2759), Heparin sodium salts (Sigma-aldrich, H3149-100KU) 0.05 mg/ml, HEPES (Life technologies, 15630106) 10 mM, GlutaMAX supplement (Life technologies, 35050061) 1X and Antibiotic-Antimycotic (Life technologies, 15240112) 1 %. 0.1 % gelatin was coated for 10 minutes at 37 ℃ incubator before seeding HUVECs on the culture dish. All cell lines were cultured in humidified 37 °C and 5 % CO2 incubator.
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