The 4% paraformaldehyde fixed tissues were embedded in paraffin and subjected to serial sections (5 um). After deparaffinizing in xylene, the sections were rehydrated in graded alcohols, and stained with hematoxylin and eosin (H&E) or used for immunohistochemistry (IHC). The primary antibody used for IHC staining was a rabbit anti-BAdV-3 polyclonal antibody (produced in our laboratory) at a dilution ratio of 1:800, and was applied overnight at 4°C. The secondary antibody was horseradish peroxidase (HRP)-labeled anti-rabbit IgG (Proteintech, China) at a dilution of 1 to 1,000 and used for 30 min at 37° C. The images of H&E and IHC were analyzed with a light microscope (Nikon, Tokyo, Japan).
Horseradish peroxidase hrp labeled anti rabbit igg
Manufactured by Proteintech
Sourced in China, United States
Horseradish peroxidase (HRP)-labeled anti-rabbit IgG is a secondary antibody conjugate used for detection in various immunoassay techniques. It is produced by linking HRP, an enzyme, to an antibody that specifically recognizes and binds to rabbit immunoglobulin G (IgG) proteins.
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Lab products found in correlation
Actinomycin d, by Beyotime (1 mentions)
Rt reaction kit, by Takara Bio (1 mentions)
High glucose dulbecco s modified eagle medium h dmem, by Thermo Fisher Scientific (1 mentions)
Rnase free dnase 1, by Takara Bio (1 mentions)
Trizol reagent, by Takara Bio (1 mentions)
Pcr primers, by Sangon (1 mentions)
2 protocols using horseradish peroxidase hrp labeled anti rabbit igg
1
Immunohistochemical Analysis of BAdV-3 in Tissue
2
ATO Modulation of Antioxidant Pathways
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ATO was purchased from Pharmaceuticals Limited Company of Harbin Medical University (Harbin, China). High-glucose Dulbecco's Modified Eagle Medium (H-DMEM) was purchased from Gibco BRL (Rockville, USA). Fetal bovine serum (FBS) was purchased from HyClone Inc. (Logan, USA). PCR primers were purchased from Sangon Biotech (Shanghai, China). RNase-free DNase I, Trizol reagent and the RT-reaction kit were purchased from TaKaRa Biotechnology (Dalian, China). Actinomycin D and TSA were from Beyotime Institute of Biotechnology (Jiangsu, China). Rabbit anti-human NFE2L2 polyclonal antibody from Signalway Antibody (Maryland, USA), Mouse anti-human Histone H3 monoclonal antibody from Tianjin Sungene Biotech Co., Ltd. (Tianjin, China), Rabbit anti-human HO-1 polyclonal antibody, mouse anti-human β-actin monoclonal antibody, horseradish peroxidase (HRP)-labeled anti-rabbit IgG and anti-mouse IgG were purchased from Proteintech Group (Chicago, USA). The enhanced chemiluminescence (ECL) kit was purchased from Pierce Biotechnology, Inc. (Rockford, USA).
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