Trypsin digestion solution

DMEM high glucose medium (lot number: 201809) was purchased from gibco; Sijiqing fetal bovine serum (lot number: 20180325) was purchased from Hangzhou Tianhang Biotechnology Co., Ltd.; (PBS) Phosphate buffer (batch number: 20180928), penicillin-streptomycin mixed solution (batch number: 201803), 0.25% trypsin digestion solution (batch number: 201710), EDTA-free trypsin digestion solution (lot number: 201901) were purchased from Solarbio, USA; Enhanced CCK-8 reagent was purchased from Shanghai Shangbao Biotechnology Co., Ltd.; (DMSO) dimethyl sulfoxide (lot number: EZ1609C224) was purchased from Biofroxx; AnnexinV-FITC/PI apoptosis double staining kit (lot number: 20181217), 1 × Binding Buffer (lot number: 20190114) were purchased from Jiangsu KGI Bio; Homo-BAX-R, Homo-BAX-F, Homo-Bcl2-R, Homo-Bcl-2-F, Homo-CASP3-R, Homo-CASP3-F, Homo-ACTB-R, Homo-ACTB-F were purchased from Shanghai Shenggong Synthetic Company; RNA extraction kit (article number: 9767, batch number: 20181124), reverse transcription kit (article number: RR036A), TB Green Premix Taq (article number: RR820) were purchased from TAKARA; absolute ethanol (article number: 64–17-5) was purchased from Guangzhou Chemical Reagent Factory; 25 cm air-permeable culture flask, 96-well plate, 6-well plate, and cryotube were purchased from corning; 0.22 μM syringe filter was purchased from Millipore.