C 660

Manufactured by Büchi

Sourced in Switzerland

The C-660 is a lab equipment product from Büchi. It is a rotary evaporator designed for solvent removal and concentration of liquid samples in laboratory settings.

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Lab products found in correlation

C 615, by Büchi (3 mentions) Smartline uv detector 2500, by Büchi (2 mentions) Sephadex lh 20, by Cytiva (2 mentions) P 1020, by Jasco (2 mentions) Silica gel, by Qingdao Marine Chemical (2 mentions) Avance 3 600, by Bruker (2 mentions) Lcms 2010ev, by Shimadzu (1 mentions) Gradient system c 605, by Büchi (1 mentions) Autospec premier p776 spectrometer, by Waters Corporation (1 mentions) Agilent 1200, by Agilent Technologies (1 mentions) Tensor 27 ftir spectrometer, by Bruker (1 mentions) C 605, by Büchi (1 mentions) C 601, by Büchi (1 mentions) Rp 18 column, by Merck Group (1 mentions) Lichroprep, by Merck Group (1 mentions) Tensor 27 infrared spectrophotometer, by Bruker (1 mentions) Drx 500, by Bruker (1 mentions)

7 protocols using c 660

Purification of Liraglutide by Preparative HPLC

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Example 4

A 5 cm MODcol column (Grace) packed with C8-bonded silica (approx. bed-depth 32 cm) was used on a preparative HPLC system (Knauer HPLC pump 1800) with detection at 220 nm (Knauer smartline UV detector 2500) and an automated fraction collector (Büchi C-660). Liraglutide purified by the two-dimensional approach given above was used as a starting material (purity: 99.2%). The column was equilibrated in Buffer A and the sample was loaded on the column at a flow rate of 43 ml/min. The detailed elution protocol is given in Table 7 below.

The purity of the pooled main fraction was 99.35% as assessed by analytical RP-UHPLC with UV detection at 220 nm. The preparation did not contain any peptidic impurity at a concentration above 0.3%.

TABLE 7

Parameters third purification dimension

Sample loading buffer3% (m/m) acetonitrile, aqueous sodium

hydrogen phosphate, pH 7.7

Buffer A3% (m/m) acetonitrile, aqueous sodium

hydrogen phosphate, pH 7.7

Buffer B61% (m/m) acetonitrile, aqueous sodium

hydrogen phosphate, pH 7.7

Elution protocol

TimeFlow rateBuffer ABuffer B

[min][ml/min][%][%]Remarks

0891000Flushing

20891000post loading

20.136.57624Elution:

10236.50100Linear gradient

US10690635B2. Purification of glucagon-like peptide 1 analogs (2020-06-23). BACHEM HOLDING AG [CH]. Inventors: Andreas Stadelmaier [DE], Ralph O. Schoenleber [CH], Daniel Samson [CH], Frank Dettner [CH].

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Silica Gel-Based Purification of Organic Compounds

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The stationary phase of separation was composed of a column Flash vide (AIT-France type) filled with a silica gel LC60A 20–45 M type. The mobile phase composed of DCM/MeOH (95:5, v/v) was pumped through the column using Büchi pump manager C-615 at a flow of 5 mL/min. The eluted fractions were collected by automatic Büchi fraction collector C-660. TLC analysis of fractions was monitored with EtOAc/MeOH (7:2, v/v).

Elloumi W., Maalej A., Ortiz S., Michel S., Chamkha M., Boutefnouchet S, & Sayadi S. (2022). Pistacia lentiscus L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities. Molecules, 27(3), 855.

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Chromatographic and Spectroscopic Analysis of Compounds

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Medium-pressure liquid chromatography (MPLC) was performed by a Buchi Gradient System C-605 apparatus using glass columns of LiChroprep® RP-18 (25–40 μm) and C-660 Buchi fraction collector. Thin-layer chromatography (TLC) performed on SiO₂ plates with n-BuOH: CH₃ COOH: H₂O (60:15:25 v/v/v) (BAW) as a mobile phase and cerium sulfate in 2N H₂ SO₄ and natural product (NP) as reagents for visualizing the spots.
H and C NMR spectra recorded by Bruker 400 MHz (H at 400 MHz and C at 100 MHz) spectrometer, using solvent signal for calibration (CD₃ OD: δH = 3.31, δC = 49.0). Distortionless enhancement by polarization transfer (DEPT) experiments was used to determine the multiplicities of C NMR resonances.
2D heteronuclear multiple bond correlation (HMBC), optimized for[2 (link)3 (link)] J_CH of 8 Hz, was used for determination of two- and three-bond heteronuclear ¹H–¹³ C connectivities while 2D heteronuclear single-quantum coherence (HSQC), interpulse delay set for ¹J_CH of 130 Hz, and COSY were used for determination of one-bond heteronuclear ¹H^–13C connectivities and homonuclear ¹H-¹H connectivities, respectively. ESIMS spectra were prepared by Shimadzu LCMS 2010 EV, using methanol as the solvent.

Chehri Z., Zolfaghari B, & Sadeghi Dinani M. (2018). Isolation of Cinnamic Acid Derivatives from the Bulbs of Allium tripedale. Advanced Biomedical Research, 7, 60.

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Detailed Analytical Techniques for Natural Product Characterization

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Optical rotations were measured on a Jasco P-1020 automatic digital polarimeter. UV spectra were obtained in an HPLC (Agilent 1200, DAD). IR spectra were obtained using a Bruker Tensor 27 FT-IR spectrometer with KBr pellets. NMR spectra were acquired with a Bruker Avance III 600 instrument at room temperature. EIMS (including HREIMS) was measured on a Waters AutoSpec Premier P776 spectrometer. Silica gel (200–300 mesh, Qingdao Marine Chemical, Inc., Qingdao, P. R. China), MCI CHP-20 (70–150 μm, Mitsubishi Chemical Corporation, Japan) and Sephadex LH-20 (Amersham Biosciences, Sweden) were used for column chromatography (CC). Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore System equipping with pump manager C-615, pump modules C-605, and fraction collector C-660 (Büchi Labor technik AG, Switzerland), and columns packed with Chromatorex C-18 (40–75 μm, Fuji Silysia Chemical Ltd., Japan). Fractions were monitored by TLC and HPLC (Agilent 1200, Extend-C18 column, 5 μm, 4.6 × 150 mm).

Gao Y., Niu Y.F., Wang F., Hai P., Wang F., Fang Y.D., Xiong W.Y, & Liu J.K. (2016). Clerodane Diterpenoids with Anti-hyperglycemic Activity from Tinospora crispa. Natural Products and Bioprospecting, 6(5), 247-255.

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Purification of Phenolic Compounds

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The PCF was separated by medium pressure liquid chromatography (MPLC) using the Büchi system consisting of a medium pressure pump (C-601), a manual injector unit, a preparative column, a UV detector (C-635) and a fraction collector (C-660). A preparative column with a diameter of 2.5 cm and a length of 50 cm and filled with modified silica gel C18 (LichroPrep, Merck 40-63 µm) was used. The column was washed first with H 2 O and then with a linear gradient of methanol-H 2 O (starting with 0 and ending with 100 % methanol). The fractions eluted from the column were collected into 15-cm 3 test tubes. The absorbance at λ = 330 nm of each collected fraction was recorded, and the phenolic profile in the tubes with the maximum and half-maximum absorbance was confirmed using the HPLC method. Samples with similar phenolic profiles were combined, and their purity was analysed using the HPLC method. Fractions containing one compound were concentrated under vacuum and dried under a stream of nitrogen. Multicomponent fractions were purified on an RP-18 column (0.8 × 25 cm, 25-40 µm, Merck) in an isocratic system (CH 3 -CN-1 % H 3 PO 4 ) with the concentration selected for each fraction on the basis of the HPLC separation. Finally, the isolated compounds were identified using the ESI-MS method.

Materska M. (2015). Flavone C-glycosides from Capsicum annuum L.: relationships between antioxidant activity and lipophilicity. European food research and technology = Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A, 240(3).

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Purification of Liraglutide by Preparative HPLC

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Example 5

A 5 cm MODcol column (Grace) packed with C8-bonded silica (approx. bed-depth 32 cm) was used on a preparative HPLC system (Knaur HPLC pump 1800) with detection at 220 nm (Knaur smartline UV detector 2500) and an automated fraction collector (Büchi C-660). Liraglutide purified by the two-dimensional approach given above was used as a starting material. The column was equilibrated in Buffer A and the sample was loaded on the column at a flow rate of 43 ml/min. The detailed elution protocol is given in Table 8 below.

TABLE 8

Parameters third purification dimension

Sample loading buffer3% (m/m) acetonitrile, aqueous disodium

hydrogen phosphate, pH 7.5

Buffer A3% (m/m) acetonitrile, aqueous disodium

hydrogen phosphate, pH 7.5

Buffer B61% (m/m) acetonitrile, aqueous disodium

hydrogen phosphate, pH 7.5

Elution protocol

TimeFlow rateBuffer ABuffer B

[min][ml/min][%][%]Remarks

0891000Flushing

20891000post loading

20.136.57624Elution:

10236.50100Linear gradient

The purity of the pooled main fraction was 99.36% as assessed by analytical RP-UHPLC. The preparation did not contain any peptidic impurity at a concentration above 0.3%.

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Analytical Characterization of Natural Products

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Optical rotations were measured on a Jasco P-1020 automatic digital polarimeter. UV data were obtained from online HPLC analysis. IR spectra (KBr) were obtained on a Bruker Tensor-27 infrared spectrophotometer. NMR spectra were acquired with a Bruker DRX-500 or Bruker Avance III 600 instrument (Bruker BioSpin GmbH, Rheinstetten, Germany) with deuterated solvent signals used as internal standards. ESI-MS (including HR-ESI-MS) were measured on API QSTAR Pulsar i mass spectrometers. Silica gel (200–300 mesh, Qingdao Marine Chemical Inc., China) and Sephadex LH-20 (Amersham Biosciences, Sweden) were used for column chromatography. Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore System equipping with pump manager C-615, pump modules C-605, and fraction collector C-660 (Büchi Labortechnik AG, Switzerland), and columns packed with Chromatorex C-18 (40–75 μm, Fuji Silysia Chemical Ltd., Japan). Fractions were monitored by TLC (Qingdao Marine Chemical Inc., China) in combination with reversed-phase HPLC (Agilent 1200, Extend-C18 column, 5 μm, 4.6 × 150 mm).

Gao Y., Zhou D.S., Hai P., Li Y, & Wang F. (2015). Hybrid Monoterpenoid Indole Alkaloids Obtained as Artifacts from Rauvolfia tetraphylla. Natural Products and Bioprospecting, 5(5), 247-253.

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