Log In Sign up
The largest database of trusted experimental protocols

206 il 010 cf

Manufactured by R&D Systems
Visit Supplier

206-IL-010/CF is a laboratory product offered by R&D Systems. It is a recombinant human Interleukin-10 protein. The core function of this product is to serve as a research tool for studying Interleukin-10, a cytokine involved in various immune and inflammatory processes.

Automatically generated - may contain errors

Lab products found in correlation

Example 16 t1165.85.2.1 cells, by R&D Systems (2 mentions) Celltiter glo reagent, by Promega (2 mentions) 485 mi 100 cf, by R&D Systems (2 mentions) Il 1β, by R&D Systems (2 mentions) Small airway epithelial cell growth medium, by PromoCell (1 mentions) Pneumocult ali medium, by STEMCELL (1 mentions)

5 protocols using 206 il 010 cf

1

Antagonism of Murine Plasmacytoma Cells by IL-6 Fab Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 16

T1165.85.2.1 cells (R&D Systems) are a murine plasmacytoma cell line that proliferates in response to mouse, rat, or human IL-6. To measure antagonism from the EBI-029 Fab mutants, 25 μL of 2 ng/mL human IL-6 (R&D Systems 206-IL-010/CF) was mixed with 25 μL of each Fab variant at a range of concentrations in a 96 well plate and incubated at RT for 30 minutes. T1165 cells in log phase were pelleted and resuspended in assay media (90% RPMI 1640, 10% FBS, 2 mM L-glutamine, Pen-Strep) at 2×105 cells/mL. 50 L of cell suspension was added to each well of IL-6/Fab mixtures to bring the final IL-6 concentration to 0.5 ng/mL. The cells were incubated at 37° C./5% C02 for 72 hours. 100 μL of Cell-Titer Glo® reagent (Promega) was added to each well and incubated at RT for 10 minutes. Luminescence was measured on a SpectraMax M5 plate reader. All mutants showed significantly greater potency compared to the wt EBI-029 Fab with no measurable IL-6 signaling over the range of Fab concentrations tested (see FIG. 7).

US10947309B2. IL-6 antibodies (2021-03-16). SESEN BIO, INC [US]. Inventors: Michael March Schmidt [US], Alison Tisdale [US], Eric Steven Furfine [US], Grigorios Zarbis-Papastoitsis [US].
+ Open protocol
+ Expand
2

Antagonism of Murine Plasmacytoma Cells by IL-6 Fab Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 16

T1165.85.2.1 cells (R&D Systems) are a murine plasmacytoma cell line that proliferates in response to mouse, rat, or human IL-6. To measure antagonism from the EBI-029 Fab mutants, 25 μL of 2 ng/mL human IL-6 (R&D Systems 206-IL-010/CF) was mixed with 25 μL of each Fab variant at a range of concentrations in a 96 well plate and incubated at RT for 30 minutes. T1165 cells in log phase were pelleted and resuspended in assay media (90% RPMI 1640, 10% FBS, 2 mM L-glutamine, Pen-Strep) at 2×105 cells/mL. 50 L of cell suspension was added to each well of IL-6/Fab mixtures to bring the final IL-6 concentration to 0.5 ng/mL. The cells were incubated at 37° C./5% C02 for 72 hours. 100 μL of Cell-Titer Glo® reagent (Promega) was added to each well and incubated at RT for 10 minutes. Luminescence was measured on a SpectraMax M5 plate reader. All mutants showed significantly greater potency compared to the wt EBI-029 Fab with no measurable IL-6 signaling over the range of Fab concentrations tested (see FIG. 7).

US11142571B2. IL-6 antibodies (2021-10-12). SESEN BIO, INC [US]. Inventors: Michael March Schmidt [US], Alison Tisdale [US], Eric Steven Furfine [US], Grigorios Zarbis-Papastoitsis [US].
+ Open protocol
+ Expand
3

Primary Mouse Neuron Cytokine Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary mouse neuronal culture was treated with IFN-γ (R&D Systems, 485-MI-100/CF) or IFN-α (R&D Systems, 12100–1) at the final concentrations of 10 ng/ml and/or 100 ng/ml was added to neuronal culture at DIV12 for 24 hrs. hAST cultures were treated with either PBS, 1 ng/mL IL-6 (R&D Systems, 206-IL-010/CF) or 10 ng/mL IL-1β (R&D Systems, 201-LB-005) for 48 hrs. After cytokine treatment, mouse neurons and hAST were either solubilized with RIPA buffer for WB or membranes were extracted for γ-secretase activity assay.
Hur J.Y., Frost G.R., Wu X., Crump C., Pan S.J., Wong E., Barros M., Li T., Nie P., Zhai Y., Wang J.C., TCW J., Guo L., McKenzie A., Ming C., Zhou X., Wang M., Sagi Y., Renton A.E., Esposito B.T., Kim Y., Sadleir K.R., Trinh I., Rissman R.A., Vassar R., Zhang B., Johnson D.S., Masliah E., Greengard P., Goate A, & Li Y.M. (2020). Innate immunity protein IFITM3 modulates γ-secretase in Alzheimer disease. Nature, 586(7831), 735-740.
+ Open protocol
+ Expand
4

Differentiation of Bronchial Stem Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Differentiation of BSCs in the ALI was previously described (21 (link), 22 (link)). Briefly, BSCs were seeded on 0.4-μm Transwell membranes in Small Airway Epithelial Cell Growth Medium (PromoCell, catalog no. C-21070). After the BSCs reached confluency, the medium was removed from the top compartment, and Pneumocult-ALI medium (StemCell Technology, catalog no. 05001) was applied in the bottom compartment. The medium was changed every 2 days. For cytokine pretreatment, BSCs in Small Airway Epithelial Cell Growth Medium were treated for 7 days with IL6 alone (10 ng/ml; R&D Systems, catalog no. 206-IL-010/CF) or a cocktail containing IL6, IFN-γ (R&D Systems, catalog no. 285-IF-100/CF), and IFNλ2 (Sigma-Aldrich, catalog no. SRP3060), each cytokine at 5 and 10 ng/ml. After treatment, BSCs were passaged (splitting ratio, 1:4) followed by differentiation in the ALI. For treatment with S3I-201 (20 μM; Selleck Chemicals, catalog no. S1155), the ALI culture was treated from Day 0 to Day 7, fixed on Day 21 when epithelial differentiation was deemed complete, and processed for cryosection and antibody staining (18 (link), 27 (link)).
Bankoti K., Wang W., Amonkar G.M., Xiong L., Shui J.E., Zhao C., Van E., Mwase C., Park J.A., Mou H., Fang Y., Que J., Bai Y., Lerou P.H, & Ai X. (2023). Airway Basal Stem Cells in COVID-19 Exhibit a Proinflammatory Signature and Impaired Mucocililary Differentiation. American Journal of Respiratory Cell and Molecular Biology, 70(1), 26-38.
+ Open protocol
+ Expand
5

Primary Mouse Neuron Cytokine Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary mouse neuronal culture was treated with IFN-γ (R&D Systems, 485-MI-100/CF) or IFN-α (R&D Systems, 12100–1) at the final concentrations of 10 ng/ml and/or 100 ng/ml was added to neuronal culture at DIV12 for 24 hrs. hAST cultures were treated with either PBS, 1 ng/mL IL-6 (R&D Systems, 206-IL-010/CF) or 10 ng/mL IL-1β (R&D Systems, 201-LB-005) for 48 hrs. After cytokine treatment, mouse neurons and hAST were either solubilized with RIPA buffer for WB or membranes were extracted for γ-secretase activity assay.
Hur J.Y., Frost G.R., Wu X., Crump C., Pan S.J., Wong E., Barros M., Li T., Nie P., Zhai Y., Wang J.C., TCW J., Guo L., McKenzie A., Ming C., Zhou X., Wang M., Sagi Y., Renton A.E., Esposito B.T., Kim Y., Sadleir K.R., Trinh I., Rissman R.A., Vassar R., Zhang B., Johnson D.S., Masliah E., Greengard P., Goate A, & Li Y.M. (2020). Innate immunity protein IFITM3 modulates γ-secretase in Alzheimer disease. Nature, 586(7831), 735-740.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!