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5 protocols using glycerol

1

SDS-PAGE and Silver Staining Protocol

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Tris-HCl, bovine serum albumin (BSA) and agarose were purchased from Hi-Media, Mumbai, India. Sodium dodecyl sulphate (SDS), ammonium per sulphate (APS), N,N,N′,N′-tetramethylethanediamine (TEMED), bromophenol blue, glycerol, acrylamide/bis, silver nitrate, sodium thiosulphate, sodium carbonate, formaldehyde (37 mL/100 mL) and Na-EDTA were purchased from SD Fine Chemicals Pvt. Ltd., Mumbai, India. Immobilized non-linear pH gradient (IPG strips) of pH 3–10 and 4–7, Bradford reagent, rehydration buffer, equilibration buffer I and II for 2D-GE were purchased from Bio-Rad, Hercules, USA. All chemicals used were of molecular biology grade. All reagents were prepared fresh during each assay.
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2

Rutin Hydrate Formulation and Characterization

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Rutin Hydrate (RUT) was procured from Sigma-Aldrich, (Burlington, MA, USA), a product of China. Phospholipid 90G was procured from Lipoid, Ludwigshafen, Germany. Sisco Research Laboratories PVT LTD, Mumbai, India provided the cholesterol, DPPH, and rhodamine-B. Other chemicals like glycerol, HPLC Water, disodium hydrogen phosphate, potassium dihydrogen phosphate and HCL were purchased from SD Fine Chemicals, (Mumbai, India). Carbopol® 934P NF (Carbopol 934P NF is used as Extended-release agent, Suspension and emulsion stabilizer and Bio adhesive agent) was received as a gift sample from Lubrizol Advanced Materials Inc., (Wickliffe, OH, USA). All the other solvents such as methanol, chloroform, ethanol, and octanol used were of laboratory grade, procured from Merck (Mumbai, India).
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3

Protocol for α-Synuclein Aggregation Assay

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The plasmid of α-synuclein was received as a kind gift from
Dr. Monica Sundd, National Institute of Immunology, New Delhi, India.
Tris buffer and copper sulfate pentahydrate were purchased from Merck.
ThT, DA hydrochloride, curcumin, and baicalein were purchased from
Sigma-Aldrich. Glycerol was purchased from SD Fine Chemicals, India,
whereas SDS was purchased from Sisco Research Laboratory, India. All
the solutions, except otherwise stated, were prepared in 20 mM Tris,
pH 7.5, and 0.1 M NaCl. Stock solutions of curcumin and baicalein
were prepared in 100% ethanol procured from Merck.
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4

Synthesis of Catalytic Nanomaterials

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Aluminum
nitrate nonahydrate
(Al (NO3)3·9H2O), magnesium
nitrate hexahydrate (Mg (NO3)2·6H2O), glycerol, zinc nitrate, glycine, titanium tetra-isopropoxide,
nitric acid (70%), DMT, and EG were purchased from S.D. Fine Chemicals,
Mumbai, India. A.R. grade chemicals were employed without any further
purification for the synthesis of catalysts and its application.
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5

Acyclovir Topical Formulation Development

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Acyclovir was a kind gift from Zen Lab & Preet Remedies Pvt. Ltd., Baddi, India.
Cellulose acetate 398-10 was brought from Sigma Aldrich Chemical, Germany; glycerol was brought from S. D. Fine Chemicals, Mumbai, India; sodium lauryl sulphate was brought from Ranbaxy Fine Chemicals Ltd., New Delhi, India. Acetone, ethanol 95% v/v, potassium dihydrogen orthophosphate, and methylene blue were obtained from S. D. Fine Chemicals, Mumbai, India.
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